A device for promoting biogas slurry return flow agitation in an intertwined biogas generation system comprises a feeding pipe, a discharging pipe and a U-shaped fermentation pipeline. The U-shaped fermentation pipeline is horizontally placed, the feeding pipe and the discharging pipe are vertically connected with two end sides of the U-shaped fermentation pipeline, a water pressure space is formed on the U-shaped fermentation pipeline, a feeding pipe drain hole is formed in the joint of the water pressure space and the feeding pipe, a discharging pipe drain hole is formed in the joint of the water pressure space and the discharging pipe, a feeding pipe gas baffle plate is arranged at the joint of the U-shaped fermentation pipeline and the feeding pipe, a discharging pipe gas baffle plate is arranged at the joint of the U-shaped fermentation pipeline and the discharging pipe, at least one gas baffle plate is arranged between the discharging pipe gas baffle plate and the feeding pipe gas baffle plate, a fermentation area is formed among the feeding/discharging pipe and the adjacent gas baffle plates or between the adjacent gas baffle plates, an independent gas collecting pipe is arranged at the top of each fermentation area, the top of each fermentation area is connected with the independent gas collecting pipe, and a valve is arranged on each gas collecting pipe. A U-shaped channel-type fermentation device is used, a fermentation path is long, biogas slurry sufficiently flows in the system by control of water pressure difference and one-way valves, bacteria are driven to be uniformly distributed, crusting is effectively prevented, and gas generating rate is high.