Method for estimating fungal biomass during a traditional solid fermentation process by using biomarker

A biomarker and solid-state fermentation technology, which is applied in biochemical equipment and methods, microbial determination/inspection, and measurement devices, can solve the problems of inability to cultivate and analyze environmental microorganisms, complicated operation process, time-consuming, etc., and achieve high promotion and application The effect of value, wide application and stable content

Inactive Publication Date: 2012-05-16
SICHUAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] (1) The present invention uses culture-free technology to analyze the fungal biomass in solid-state fermentation samples, avoiding the time-consuming and complicated operation process of traditional culture methods, and solving the analysis errors caused by the inability to cultivate a large number of environmental microorganisms

Method used

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  • Method for estimating fungal biomass during a traditional solid fermentation process by using biomarker

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Embodiment 1

[0013] Collect the solid-state fermentation sample used in the experiment, pulverize and mix well, weigh 1g of the sample, add 10ml of methanol, and sonicate for 5min; Then add an appropriate amount of distilled water for dilution; the obtained saponified solution is extracted three times with 120ml of n-hexane, the organic phase is collected, and the n-hexane is removed by distillation under reduced pressure; (HPLC) analysis and determination; in combination with relevant data processing methods, analyze and obtain the ergosterol content of the sample, and estimate the fungal biomass of the sample accordingly. According to Example 1, the biomass of fungi in medium-high temperature koji used for brewing in a well-known liquor factory is about 188.69±2.3μg·mg -1 , the fungal biomass in high-temperature koji is about 110.45±4.6μg·mg -1 .

Embodiment 2

[0015] Collect the solid-state fermentation sample used in the experiment, pulverize and mix well, weigh 1g of the sample, add 10ml of methanol, and sonicate for 10min; After that, add an appropriate amount of distilled water for dilution; the obtained saponified solution is extracted 3 times through 90ml toluene, and the organic phase is collected, and the toluene is removed by distillation under reduced pressure; ) analysis and determination; in combination with relevant data processing methods, the ergosterol content of the sample is obtained by analysis, and the fungal biomass of the sample is estimated accordingly. According to Example 2, the biomass of fungi in vinegar koji used for making vinegar in a well-known bran vinegar factory is about 119.63±6.07 μg·mg -1 .

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Abstract

The invention discloses a culture-free biochemical method for estimating fungal biomass during a traditional solid fermentation process based on an ergosterol biomarker. The method comprises steps that: (1) a sample is pre-treated; (2) saponification is carried out; (3) a sample saponification liquid is extracted; (4) HPLC detection of ergosterol is carried out; and (5) the sample fungal biomass is analyzed and estimated. The method provided by the invention assists in providing fast and accurate analysis means for the researches of bacterial community structural compositions of traditional solid fermentation systems, and especially for the tracking of variation trends of the fungal biomass during the fermentation processes. The method provided by the invention assists in providing a theoretical basis for scientific regulation of traditional solid fermentation process. The method provides good social and economic benefits.

Description

technical field [0001] The invention belongs to the field of non-culture analysis of microbial community structure in solid-state fermentation process. Ergosterol is the main sterol component of most fungal cells with stable content and is a reliable biomass marker. Based on the analysis results of sample ergosterol, it can Rapid estimation of fungal biomass in traditional solid-state fermentation processes. Background technique [0002] Solid-state fermentation originated in China and has a history of thousands of years. Due to its unique advantages of saving water and energy, solid-state fermentation is a clean production technology, and it has gradually attracted the attention of countries all over the world in recent years. Traditional solid-state fermented foods in my country, such as liquor, vinegar, soy sauce, watercress, fermented bean curd, etc., have complex production processes and mostly use natural enrichment inoculation. The unique production process and long p...

Claims

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Application Information

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IPC IPC(8): C12Q1/06G01N30/02
Inventor 周荣清秦臻郑佳黄钧
Owner SICHUAN UNIV
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