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Methods for producing glucosamine from microbial biomass

A kind of technology of glucosamine and microbial biomass, applied in the production field of glucosamine

Inactive Publication Date: 2009-09-23
技源集团有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The present invention is developed for the current state of the process, especially for the problems and needs that have not been fully resolved in the existing glucosamine production methods

Method used

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  • Methods for producing glucosamine from microbial biomass
  • Methods for producing glucosamine from microbial biomass
  • Methods for producing glucosamine from microbial biomass

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 100 g of dried citric acid biomass was mixed with 250 ml of 20% hydrochloric acid, and the mixture was heated to reflux at three different temperatures in three experiments: 60 °C, 80 °C and 100 °C. Samples were taken at different intervals, and the reaction was analyzed by high-performance liquid chromatography. The chromatograph model was "Agilent 1100" of Agilent Company. See the reaction result Figure 4 , which shows the production of glucosamine as a function of reaction time and temperature. As shown, starting with 100 grams of dry biomass (which contains about 10 grams of chitin), the reaction produced about 6 grams of glucosamine. Thus, relative to the starting 100 grams of dry biomass, the yield was 6%; relative to 10 grams of chitin in the dry biomass, the yield was 60%.

[0044] It can be seen from the figure that the yield of glucosamine obtained by the reaction at 80°C and 100°C is higher than that at 60°C. It is obvious that the yield of glucosamine in...

Embodiment 2

[0048] 100 g of dry citric acid biomass was mixed with 300 ml of 31% hydrochloric acid, and the mixture was heated to reflux at 100° C. for 2.5 hours. After the reaction, filter and evaporate the filtrate to about 15-30 ml. About 50 ml of ethanol was added to this solution, and the mixture was vortexed to accelerate the precipitation of glucosamine. Then, the precipitated glucosamine was filtered from the solution and washed with ethanol. Dissolve the precipitated glucosamine in water and recrystallize with an evaporator. The purity of glucosamine in the final product is greater than 98%.

Embodiment 3

[0050] 100 grams of dried mushrooms were mixed with 300 milliliters of 31% hydrochloric acid, and the mixture was heated to reflux at 100° C. for 2.5 hours. After the reaction was complete, the mixture was filtered and the filtrate was evaporated to about 15-30 ml. About 50 ml of ethanol was added to this solution, and the mixture was vortexed to accelerate the precipitation of glucosamine. Then, the precipitated glucosamine was filtered from the solution and washed with ethanol. Dissolve the precipitated glucosamine in water and recrystallize with an evaporator. The purity of glucosamine in the final product is greater than 98%.

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Abstract

A more efficient method for producing glucosamine from microbial biomass such as, for example, fungal biomass is disclosed. In one embodiment, a method for obtaining glucosamine from fungal biomass includes providing fungal biomass containing chitin, such as Aspergillus, Penicillium, Mucor, or mushroom. The fungal biomass is then reacted in an acidic solution with an acid concentration of greater than about 20% by weight and at a reaction temperature greater than about 60 DEG C to convert the chitin to glucosamine. This reaction is performed for a period of less than 4 hours. The glucosamine is then separated from the acidic solution. In selected embodiments, dried fungal biomass is used to accelerate the reaction. This dried fungal biomass may, in some embodiments, have a moisture content of less than about 20% and in some cases less than about 13%.

Description

field of invention [0001] The present invention relates to the production of glucosamine, in particular to methods for the more efficient production of glucosamine from microbial biomass, such as fungal biomass. Background technique [0002] Glucosamine is a molecule containing glucose and amines that is naturally synthesized in the body to promote cartilage tissue health. The glucosamine molecule is thought to encourage chondrocytes to produce two proteins, proteoglycan and collagen, that help hold joint tissue together. Glucosamine is usually not ingested directly from food, but is synthesized in the body by the body using ingredients in food. [0003] As we age, the body gradually loses the ability to synthesize glucosamine, thus reducing the elasticity of cartilage and making it more susceptible to damage. People with arthritis are more typical of cartilage breakdown and slow synthesis. To compensate for this effect, glucosamine is widely used as a supplement to provi...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07H5/06C07H1/08
CPCC12P19/26A61P19/00A61P19/02
Inventor 曹礼群蒋永红余远明魏献忠李五洲
Owner 技源集团有限公司
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