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Method for online in-situ monitoring of solid-state fermentation fungus biomass

A solid-state fermentation and biomass technology, which is applied in microorganism-based methods, biochemical equipment and methods, and microbial determination/inspection, etc., can solve the problem that biomass cannot be accurately measured online, and achieve strong repeatability and little impact. , the effect of measuring a wide range

Inactive Publication Date: 2012-03-28
INST OF PROCESS ENG CHINESE ACAD OF SCI
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Problems solved by technology

[0006] Aiming at the problem that the biomass in the solid-state fermentation process cannot be accurately measured online, the patent of the present invention provides an on-line in-situ monitoring method for the biomass of solid-state fermentation fungi, specifically referring to an application of in-situ digital image analysis technology combined with modern chemometric methods Method for the Non-Destructive Determination of Fungal Biomass in Solid State Fermentation

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  • Method for online in-situ monitoring of solid-state fermentation fungus biomass
  • Method for online in-situ monitoring of solid-state fermentation fungus biomass

Examples

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Embodiment 1

[0022] Example 1 On-line in-situ monitoring of biomass changes in the solid-state fermentation process of Trichoderma viride

[0023] Trichoderma viride is the main production strain of cellulase produced by solid-state fermentation, and the fermentation substrate is lignocellulosic material. In this embodiment, the steps for on-line in-situ monitoring of Trichoderma viride solid-state fermentation to produce cellulase are as follows:

[0024] 1. Modeling sample preparation. Use steam-exploded rice straw and wheat bran (ratio: 4:1) as the solid-state fermentation medium, adjust the water content of the medium to about 75%, and inoculate 10% Trichoderma viride spore liquid (concentration: 5 × 108 / mL ), cultured for 7 days at a temperature of 30°C and a humidity of 98%, and 50 groups of parallel experiments were done. Three groups of fermented products were taken every 12 hours and photographed with a high-resolution digital camera, and the biomass in the fermented products wa...

Embodiment 2

[0040] Example 2 On-line in-situ detection of biomass changes during solid-state fermentation of Aspergillus niger

[0041] Aspergillus niger is the main production strain of α-acid amylase by solid-state fermentation. The fermentation substrate is 85% bran, 10% corn starch, 5% bean cake powder, and the ratio of material to water is 1:1. In this embodiment, the steps for on-line in situ monitoring of Aspergillus niger solid-state fermentation to produce α-acid amylase are as follows:

[0042] 1. Modeling sample preparation. With 85% bran, 10% corn starch, and 5% bean cake powder as the solid-state fermentation medium, adjust the water content of the medium at about 60%, and inoculate 10% Aspergillus niger spore liquid (concentration is 5 × 108 / mL ), cultured for 5 days at a temperature of 30°C and a humidity of 98%, and 50 groups of parallel experiments were done. Three bottles of fermented product were taken every 12 hours and photographed with a high-resolution digital cam...

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Abstract

The invention relates to a method for online in-situ monitoring of solid-state fermentation fungus biomass, which is characterized in that the method comprises the following steps of: carrying out fermentation test outside a reactor by using a production strain, shooting thalluses-matrix images at different fermentation stages, obtaining fractal dimension through digital image processing, simultaneously determining corresponding thallus biomass by using a chemical method and calculating a functional relation between the fractal dimension and the thallus biomass; shooting an thallus growth image in the solid-state fermentation process by using a built-in camera in the reactor, calculating the fractal dimension of the thallus growth image and obtaining corresponding biomass according to a functional relation between the fractal dimension and the biomass. Through the method, the online monitoring of the solid-state fermentation fungal biomass can be realized, and the automatic solid-state fermentation control can be promoted.

Description

technical field [0001] The patent of the invention belongs to the field of on-line in-situ monitoring of solid-state fermentation biomass, and specifically refers to a method for non-destructive determination of solid-state fermentation fungal biomass using in-situ digital image analysis technology combined with modern chemometric methods. Background technique [0002] Determining the biomass change of production strains in the fermentation process is the basis for establishing the growth kinetics of solid-state fermentation microorganisms. It is of great significance for the study of microbial growth control in the fermentation process and optimization of the fermentation process. Therefore, it has always been an important content of fermentation kinetics research. The production strains of solid-state fermentation are mostly filamentous fungi. During the fermentation process, the mycelium and the substrate are entangled and adhered, and the two are difficult to separate. Th...

Claims

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Application Information

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IPC IPC(8): C12Q1/06C12R1/885C12R1/685
Inventor 陈洪章段颖异
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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