The invention discloses gloeostereum incarnatum cattle stomach rich in gloeostereum incarnatum polysaccharide and a preparation method thereof. The gloeostereum incarnatum cattle stomach rich in gloeostereum incarnatum polysaccharide comprises the following components: gloeostereum incarnatum, hot pepper, soybean oil, soybean protein isolate, white granulated sugar, Chinese prickly ash, garlic slices, cinnamon, star anise, fennel, liquorice, ginger, table salt, soy sauce, gloeostereum incarnatum polysaccharide, carrageenan, cellulase, monosodium glutamate, disodium nucleotide and sodium diacetate. The preparation method comprises the following steps: S1, preparing gloeostereum incarnatum powder; S2, preparing gloeostereum incarnatum fibers by an enzyme method; S3, forming gloeostereum incarnatum cattle stomach; S4, preparing seasoning liquid; S5, chili oil preparation; S6, mixed seasoning; S7, baking and stir-frying; and S8, packaging and sterilization. The raw materials of the present invention are a medicinal fungus with an appearance similar to that of a tripe, and is a high-protein and low-fat food. The mouthfeel of the sanguisorba is similar to that of a tripe, is crispy and refreshing, and is rich in sanguisorba polysaccharide in the Sanguisorba, and has good antioxidant, bacteriostasis and anti-tumor activity. The raw materials of the present invention are prepared into the gloeostereum incarnatum, so that the development of edible mushroom resources is facilitated.