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Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum

A technology for Fusarium graminearum and plant essential oil, which is applied in the field of preparation of plant essential oil emulsion, can solve the problems of small droplet aggregation, limited capacity, flocculation and sedimentation, etc., achieves good bacteriostatic effect, good inhibitory effect, and overcomes poor water solubility. Effect

Active Publication Date: 2020-04-17
JIANGNAN UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] However, direct emulsification of essential oils often leads to unstable phenomena such as small droplets converging into large droplets to form flocculation and precipitation. The oil-in-water emulsion delivery system can significantly improve the water solubility of essential oils
However, other oil phases such as corn oil or MCT introduced in the emulsion preparation process will weaken the antibacterial effect of the essential oil emulsion, and the ability of the emulsifier to wrap the oil phase is limited, so to improve the antibacterial ability of the emulsion, it is necessary to increase the antibacterial effect of the essential oil. Ratio, reducing the introduction of other oils

Method used

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  • Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum
  • Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum
  • Preparation method of plant essential oil emulsion for inhibiting growth of fusarium graminearum

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Effect test

Embodiment 1

[0031] Embodiment 1 prepares clove oil emulsion

[0032] (1) Take 8mL of clove oil as the oil phase, dissolve the lecithin with a concentration of 1wt% in 10mM acetate buffer, and filter through a double-layer filter cloth to obtain an emulsifier solution;

[0033] (2) Take 92mL of the emulsifier solution prepared in step (1) and 8mL of clove oil and mix them. The mixing system is 100mL. Use a hand-held homogenizer to homogenize at 32000rpm for 2min, and then pass through a high-pressure homogenizer under a pressure of 1000bar. Circulate and homogenize at a rate of 9 L / h for 1 min to obtain clove oil emulsion.

Embodiment 2

[0034] Embodiment 2 prepares litsea cubeba oil emulsion

[0035] (1) Get 8mL litsea cubeba oil as the oil phase, dissolve the lecithin with a concentration of 1wt% in 10mM acetate buffer, and filter through a double-layer filter cloth to obtain an emulsifier solution;

[0036] (2) 92mL of the emulsifier solution prepared in step (1) and 8mL of Litsea cubeba oil were mixed, and the mixing system was 100mL, and was homogenized for 2min at 32000rpm using a hand-held homogenizer, and then passed through a high-pressure homogenizer under pressure. Circulation and homogenization at a rate of 9 L / h for 1 min at 1000 bar to obtain litsea cubeba oil emulsion.

Embodiment 3

[0037] Embodiment 3 changes homogeneous condition

[0038] (1) Get 8ml clove oil and 8ml litsea cubeba oil respectively as the oil phase, dissolve lecithin with a concentration of 1wt% in 10mM acetate buffer, and filter with a double-layer filter cloth to obtain an emulsifier solution;

[0039] (2) Take the emulsifier solution prepared in step (1), mix it with 8mL clove oil and 8mL Litsea cubeba oil respectively, the mixed system is 100mL, use a hand-held homogenizer to homogenize at 32000rpm for 2min, and then use 9L / Through the high-pressure homogenizer at a speed of h, a series of clove oil emulsion and litsea cubeba were obtained under five pressures (400, 700, 1000, 1300 and 1600bar) and three homogenization times (0.5, 1 and 1.5min). oil emulsion.

[0040] The average particle size of the emulsion obtained after different homogenization pressure and different homogenization time is shown in Table 1. The particle size distribution of clove oil emulsion and litsea cubeb...

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Abstract

The invention discloses a preparation method of a plant essential oil emulsion for inhibiting growth of fusarium graminearum, which belongs to the technical field of food preservation. According to the method, plant essential oil is used as an oil phase, lecithin is used as an emulsifier, an acetate buffer solution is used as a water phase, and an emulsification method combining high-speed shearing and high-pressure homogenization is utilized to prepare the essential oil emulsion for inhibiting fusarium graminearum growth without adding an Ostwald ripening inhibitor. The plant essential oil emulsion prepared by the invention is uniform in dispersion and has relatively good physical stability. Meanwhile, other oil phases such as corn oil or MCT are not introduced into the plant essential oil emulsion disclosed by the invention; the plant essential oil disclosed by the invention can achieve a relatively good antibacterial effect at a relatively low concentration; when the emulsion concentration of the plant essential oil emulsion prepared from clove oil is 40 mL / L, the inhibition rate of fusarium graminearum is 75.7%, and a new method is provided for controlling fungal biomass and mycotoxin in the grain storage and processing process.

Description

technical field [0001] The invention relates to a preparation method of a plant essential oil emulsion that inhibits the growth of Fusarium graminearum, and belongs to the technical field of food antisepsis and preservation. Background technique [0002] At present, grains such as wheat, barley and oats are generally polluted by scab caused by Fusarium. affect the quality and yield of agricultural products. Among them, Fusarium graminearum (Fusarium graminearum) is a main strain that causes head blight, and it will produce toxins such as deoxynivalenol in the field and in the grain storage process, causing serious damage to people's life and health. threaten. At present, researchers are developing various new green antibacterial and antifungal agents to reduce the negative effects of traditional antifungal methods (radiation, chemical fungicides, etc.). [0003] Essential oils (EOs) are natural antimicrobial agents produced by many plants to protect themselves from infect...

Claims

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Application Information

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IPC IPC(8): A23L3/3472
CPCA23L3/3472A23V2002/00A23V2200/10A23V2250/1842
Inventor 吴殿辉王璐冯文旭陆健蔡国林翟秀超
Owner JIANGNAN UNIV
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