Liquid strain of edible mushrooms and method for producing liquid strain of edible mushrooms

A production method and technology of liquid strains, applied in botany equipment and methods, applications, gardening, etc., can solve the problems of unsuitable shiitake mushrooms, difficult pollution, weak growth of strains, etc., to achieve long-term preservation and transportation, reduce Production cost and the effect of improving production efficiency

Inactive Publication Date: 2014-10-22
保山富群农业科技有限公司
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Problems solved by technology

Although liquid strains have overcome the defects of long production cycle and high cost of traditional solid strains, there are still a series of problems that are not suitable for large-scale industrialization of shiitake mushrooms, such as large investment in liquid fermentation production equipment, difficult pollution control, rapid mycelium aging, and strain variation. homework defects
[0004] In the existi

Method used

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  • Liquid strain of edible mushrooms and method for producing liquid strain of edible mushrooms

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preparation example Construction

[0051] The preparation method of the culture medium used in the embodiment is, the Moringa oleifera sawdust (the part between 20-40 meshes of sawdust particles) and the nutrient solution are mixed and prepared in a weight ratio of 1:1.2, and the formula of the nutrient solution is prepared with 10% The obtained bran water was incubated at 80-93° C. for 1 hour and then filtered with 4 layers of gauze. Add 2 grams of sucrose, 0.2 grams of potassium dihydrogen phosphate, and 0.1 grams of magnesium sulfate to each 100 ml of filtrate to adjust the pH to 6.0. Then the medium was sterilized at 121°C for 30 minutes, and then cooled to 30°C under sterile conditions for use.

[0052] The detection of the content of solid strains in liquid strains is carried out in accordance with the method specified in NY / T1742-2009, General Technical Requirements for Edible Fungi Strains.

[0053] The detection method of mycelial germination rate is as follows: the liquid strain of edible fungus is co...

Embodiment 1

[0056] This embodiment is used to illustrate the method for producing edible fungus liquid strains provided by the present invention.

[0057] Inoculate the mushroom strain (Qingke 20) into PDA medium for activation and culture to obtain mycelium; then transfer the mycelium to solid wood chip medium at a ratio of 4%, and place it at 25°C for fermentation and culture for 30 1 day, obtain 100g mycelium block culture; Under sterile conditions, the block culture is smashed into the powdery granule culture of particle size not more than 0.5cm fritter, the powdery granule culture is with 3 The thickness of -4cm is tiled in the culture tray, sealed, protected from light, and cultured at a constant temperature of 25°C for 3 days; Add 250ml of sterile water at 5000rpm / min and beat for 30s at a rotating speed of 5000rpm / min to prepare a mother liquor, and then mix 4750ml of agar solution with a concentration of 0.2% with the prepared mother liquor to obtain 5000ml of shiitake mushroom l...

Embodiment 2

[0059] This embodiment is used to illustrate the method for producing edible fungus liquid strains provided by the present invention.

[0060] The same method as in Example 1 was used to prepare the liquid spawn of mushrooms, except that the amount of 0.2% agar solution added was 4500 ml.

[0061] Obtain the mushroom liquid dilution strain 2.

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Abstract

The invention discloses a method for producing a liquid strain of edible mushrooms. The method for producing the liquid strain of the edible mushrooms comprises the steps that (1) the liquid strain of the edible mushrooms is inoculated in a PDA culture medium to be activated, and separation is conducted after cultivation so that an activated mycelium can be obtained; (2) the activated mycelium is inoculated in a solid-state saw dust culture medium to be cultivated for 28-32 days so that a recovery culture 1 composed of the mycelium and the solid-state saw dust culture medium can be obtained; (3) the recovery culture 1 is smashed into particles with the diameters ranging from 20 meshes to 40 meshes and then the particles are laid flat in a shallow plate with a thickness of 3 m-4m to be cultivated for 2-3 days in a sealed mode so that a recovery culture 2 can be obtained; (4) after the recovery culture 2 is smashed into small blocks with the diameter not larger than 0.5 cm, pulping is conducted by adding water to the small blocks, and then the obtained pulp and the agar solution with the concentration ranging from 0.15% to 0.2% are made to be in contact and evenly mixed. According to the method for producing the liquid strain of the edible mushrooms, operation is easy, a complicated cultivation device is not needed, and the production efficiency is high. The liquid strain, obtained according to the method, of the edible mushrooms has the advantages of being stable in quality, robust, slow in degeneration and easy to store and transport.

Description

technical field [0001] The invention relates to a liquid strain of edible fungus and a production method of the liquid strain. The production method of the liquid strain of edible fungus. Background technique [0002] In the existing edible fungus cultivation technology, solid strains are usually used for production. Traditional solid strains have the defects of long production cycle of edible fungi strains, large amount of strains, high production cost, and slow mycelial germination speed. It is not suitable for the application of shiitake mushrooms in large-scale production. [0003] The liquid strains commonly used in the prior art are produced by submerged culture techniques in biological fermenters using liquid culture medium. Although liquid strains have overcome the defects of long production cycle and high cost of traditional solid strains, there are still a series of problems that are not suitable for large-scale industrialization of shiitake mushrooms, such as lar...

Claims

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Application Information

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IPC IPC(8): A01G1/04
Inventor 黄伟
Owner 保山富群农业科技有限公司
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