A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking

A technology of rabiglutin and cauliflower, which is applied in the field of botany, can solve the problems of low rabigluside content, poor anti-cancer and anti-cancer functions, etc., and achieve the effect of significant technological progress, small workload, and avoiding long cycle times

Inactive Publication Date: 2017-08-15
SHANGHAI ACAD OF AGRI SCI +1
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Problems solved by technology

[0005] Aiming at the above-mentioned technical problems in the prior art, the method for preparing a new germplasm of cauliflower rich in radhurin by using molecular markers described herein should solve the problem that the content of radradin in the cauliflower in the prior art is very low, and the anti-cancer Technical problems with poor anti-cancer function

Method used

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  • A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking
  • A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking
  • A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking

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Embodiment 1

[0017] Example 1: Obtained by screening of SSR markers and InDel markers related to rapaside synthesis genes

[0018] 1. Acquisition of SSR mark

[0019] 1.1 Materials

[0020] Broccoli high-generation inbred lines LBS and LI and their hybrid F2 populations, the highest content of rabioside in LBS was 7.16 μmol / g dry weight, and the lowest content in LI was 0.24 μmol / g dry weight.

[0021] 1.2 Establishment of gene pools with high and low ratin content

[0022] For all single plants of the F2 generation, during the commercial curd harvest period, the commercial curds are mixed and sampled by the three-point method (that is, the top and the edge of the ball, a total of 3 points), vacuum freeze-dried, crushed evenly with a mill, and stored in a -18°C refrigerator save. The content of rabiglutin in curds of all F2 generation single plants was determined by reversed-phase high-performance liquid chromatography. According to the measurement results, select 5 individual plants w...

Embodiment 2

[0045] Example 2: Using molecular markers to prepare new germplasm of cauliflower rich in rabioside

[0046] 1) According to the results of the determination of rabiglutin content, the broccoli inbred line LBS (content 7.16 μmol / g dry weight) with a high rapain content was selected and the cauliflower inbred line 830 was configured to cross F 1 generation seed, F 1 F 2 generation seed, sow F 2 generation seed gets F 2 Generation plant;

[0047] 2) F as described in step 1 2 The single plants whose curd, plant type and leaf shape tend to be cauliflower were selected among the generation plants, and the first prospect selection was carried out on these single plants by using the SSR markers, ELONG-InDel and ALK-InDel markers related to the ratin synthesis gene , obtained the same F 2 Generation single plant, with the corresponding broccoli parent 830 as the recurrent parent, to obtain BC 1 f 2 generation seed, sow BC 1 f 2 generation seed to get BC 1 f 2 Generation p...

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Abstract

A method of preparing a novel cauliflower variety rich in glucoraphanin by utilizing molecular marking is disclosed. Through a process of crossing, selfing and back-crossing in order, based on a molecular marking technique, and through combining marker assisted selection and traditional phenotypic selection, related gene fragments in a broccoli selfing line having a high glucoraphanin content are transferred to a cauliflower selfing line having excellent comprehensive properties to obtain the novel cauliflower variety having a high glucoraphanin content. By utilizing molecular marking, single plants having high glucoraphanin contents can be selected in a seedling stage and single plants having low glucoraphanin contents are eliminated, the area of land utilized is small, the working load is low, and disadvantages such as long cycles, high working loads and susceptibility to environment influences of methods for measuring glucoraphanin contents of ball-flowers in conventional breeding methods can be overcome. Different cauliflower selfing lines can be subjected to high-glucoraphanin-content creation so that passive glucoraphanin content breeding is turned into initiative breeding and creation, thus providing rich original materials for breeding the novel cauliflower variety having the high glucoraphanin content.

Description

technical field [0001] The invention belongs to the field of botany and relates to a cauliflower, in particular to a method for preparing a new germplasm of the cauliflower rich in rabiglutin by using a molecular marker. Background technique [0002] With the development of modern industry and the intensification of environmental pollution, the incidence of cancer in my country has continued to rise in the past 10 years, and the total cancer death rate has increased by 31%. Among them, lung cancer, liver cancer, breast cancer, colon cancer, rectal cancer and other cancers closely related to lifestyle are rising rapidly, especially in cities. Therefore, research on the relationship between anticancer substances in vegetables and common human cancers has attracted more and more attention from scientists. A large number of medical and nutritional studies at home and abroad have shown that regular intake of cruciferous vegetables such as broccoli can effectively reduce the inci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
CPCA01H1/02A01H1/04
Inventor 姚雪琴谢祝捷李光庆刘春琴
Owner SHANGHAI ACAD OF AGRI SCI
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