The invention relates to a method for extracting and purifying
sesquiterpene coumarin aether in the field of
chemical technology, which comprises the steps as follows: step 1: southernwood roots are smashed and ultrasonically extracted with
methanol; the extracting solution is rotary-evaporated and condensed to obtain extract which is then delaminated by adopting acetic
ether and water double-phase dispersion and extraction; the acetic
ether organic phase of an upper layer is taken as an extracting concentrate; step 2:
column chromatography is carried out after the extracting concentrate is added with an adsorbent;
elution is carried out by using
petroleum ether and acetic ether mixed eluant, and then thin-layer
chromatography is carried out to collect components containing the
sesquiterpene coumarin aether; step 3: the concentrate is furthered condensed to the extract which is then dispersed with
methanol of same volume and then filtered with a nylon membrane, purified with the reversed-phase column of a preparative
liquid phase ODS RP-C18 and eluted with mixture solution of
methanol and water; and finally, the corresponding components are condensed. The invention has simple preparation method and easily available raw materials for preparation and can be applied to
chemoprophylaxis and cure of tumors and development of relevant antitumor drugs.