Pharmaceutical composition and application thereof

A composition and drug technology, applied in the field of medicine, can solve the problems of limited effect of preventing and treating tumors, and achieve the effects of significant synergy and synergy, inhibiting the growth and metastasis of tumor cells, and inhibiting the occurrence and development of liver tumors.

Active Publication Date: 2020-10-13
SHAANXI GIANT BIOTECHNOLOGY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0015] In order to solve the problem of limited effect of preventing and treating tumors caused by using ginsenoside monomer alone or using bicyclol alone in the prior art, the application provides a synergistic antitumor pharmaceutical composition, which can be safer and more effective. Potent prevention and treatment of tumors, especially liver cancer

Method used

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  • Pharmaceutical composition and application thereof
  • Pharmaceutical composition and application thereof
  • Pharmaceutical composition and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0076] Example 1 Combination of ginsenoside CK and bicyclol inhibits the proliferation of liver cancer HepG2 cells in vitro

[0077] In this example, the MTT method was used to detect the inhibitory effect of ginsenoside CK on the proliferation of liver cancer HepG2 cells. The chemical name of "MTT" is 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, which is a yellow dye. "MTT method", also known as "MTT colorimetric method", is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal formazan (Formazan) and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its absorbance value is measured at a wavelength of 490nm with a microplate reader. Within a certain range of cell numbers, the amount of MTT crystal formation is proportional to the number of...

Embodiment 2

[0084] Example 2 The combination of ginsenoside CK and bicyclol inhibits the malignant transformation of WB-F344 cells induced by 3MC / TPA into cancer cells in vitro

[0085] The models for studying tumor chemoprevention drugs mainly include in vitro system and in vivo system. The in vitro system uses various carcinogens to directly or indirectly induce malignant transformation of normal cells. The effective indicator is the inhibition of malignant transformation of cells. It has the advantages of being able to simulate the multi-stage process of tumorigenesis in vivo and the experimental conditions are easy to control. Rat liver epithelial-like stem cells (WB-F344 cells) come from normal adult isogenic male rats. They are immature liver epithelial cells with the properties of liver stem cells. They have a certain relationship with the occurrence of liver cancer and are liver cancer precursor cells. .

[0086] Model group: 1500 WB-F344 cells / well were seeded in 6-well plates, ...

Embodiment 3

[0094] Example 3 In vivo efficacy evaluation of ginsenoside CK and bicyclol in the treatment of liver cancer

[0095] Establishment of human liver cancer xenograft tumor model in nude mice: female BALB / c nude mice aged 4-5 weeks were subcutaneously inoculated with Hep-3B cells in the left axilla, and each nude mouse was injected with 4×10 6 cells. Tumor volume reaches 100-300mm 3 , the nude mice were randomly divided into 16 groups, with 10 animals in each group.

[0096] The 16 groups were 1 model group and 15 drug-dosing groups, and the 15 drug-dosing groups were: (1) 5 ginsenoside CK groups, administered by intragastric administration, the dosages were 60mg / kg and 30mg / kg respectively . kg; (3) 5 ginsenoside CK / bicyclol combination groups, intragastric administration, the doses of ginsenoside CK / bicyclol were 60mg / kg / 60mg / kg, 30mg / kg / 90mg / kg, 90mg / kg / 30mg / kg, 100mg / kg / 20mg / kg, 24mg / kg / 96mg / kg. The administration volume of each dosing group was 0.2 mL, administered onc...

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Abstract

The invention discloses a pharmaceutical composition. The pharmaceutical composition is characterized by comprising a ginsenoside monomer and bicyclol. The pharmaceutical composition has a remarkablecurative effect on chemoprevention and treatment of liver cancer, and has remarkable synergistic and synergistic effects compared with the ginsenoside monomer or bicyclol used alone. The invention further discloses application of the ginsenoside monomer and bicyclol in preparation of the pharmaceutical composition for preventing and / or treating tumors and application of the ginsenoside monomer inpromotion of the effect of the pharmaceutical composition for preventing and / or treating tumors.

Description

technical field [0001] The application belongs to the technical field of medicine, in particular, the application relates to a pharmaceutical composition and its application. Background technique [0002] Primary liver cancer is one of the most common malignant tumors worldwide. The National Cancer Center estimates that in 2015, the number of cases of liver cancer in China is 466,000, and the number of deaths from liver cancer is 422,000. Liver cancer is currently the fourth most common malignant tumor and the third leading cause of cancer death in my country, seriously threatening the lives and health of our people. [0003] Patients with early liver cancer generally have no obvious symptoms, and most patients have already developed intermediate or advanced liver cancer when they see a doctor. The average survival time of untreated primary liver cancer after diagnosis is less than 4 months. Currently, the best treatments for liver cancer are surgical resection and liver tr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/704A61K31/7032A61K31/575A61K31/36A61P35/00A61P1/16
CPCA61K31/704A61K31/7032A61K31/575A61K31/36A61P35/00A61P1/16A61K2300/00
Inventor 范代娣殷诗玉段志广马晓轩
Owner SHAANXI GIANT BIOTECHNOLOGY CO LTD
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