Pharmaceutical composition and application thereof
A composition and drug technology, applied in the field of medicine, can solve the problems of limited effect of preventing and treating tumors, and achieve the effects of significant synergy and synergy, inhibiting the growth and metastasis of tumor cells, and inhibiting the occurrence and development of liver tumors.
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Embodiment 1
[0076] Example 1 Combination of ginsenoside CK and bicyclol inhibits the proliferation of liver cancer HepG2 cells in vitro
[0077] In this example, the MTT method was used to detect the inhibitory effect of ginsenoside CK on the proliferation of liver cancer HepG2 cells. The chemical name of "MTT" is 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide, which is a yellow dye. "MTT method", also known as "MTT colorimetric method", is a method for detecting cell survival and growth. The detection principle is that succinate dehydrogenase in the mitochondria of living cells can reduce exogenous MTT to water-insoluble blue-purple crystal formazan (Formazan) and deposit in the cells, while dead cells have no such function. Dimethyl sulfoxide (DMSO) can dissolve formazan in cells, and its absorbance value is measured at a wavelength of 490nm with a microplate reader. Within a certain range of cell numbers, the amount of MTT crystal formation is proportional to the number of...
Embodiment 2
[0084] Example 2 The combination of ginsenoside CK and bicyclol inhibits the malignant transformation of WB-F344 cells induced by 3MC / TPA into cancer cells in vitro
[0085] The models for studying tumor chemoprevention drugs mainly include in vitro system and in vivo system. The in vitro system uses various carcinogens to directly or indirectly induce malignant transformation of normal cells. The effective indicator is the inhibition of malignant transformation of cells. It has the advantages of being able to simulate the multi-stage process of tumorigenesis in vivo and the experimental conditions are easy to control. Rat liver epithelial-like stem cells (WB-F344 cells) come from normal adult isogenic male rats. They are immature liver epithelial cells with the properties of liver stem cells. They have a certain relationship with the occurrence of liver cancer and are liver cancer precursor cells. .
[0086] Model group: 1500 WB-F344 cells / well were seeded in 6-well plates, ...
Embodiment 3
[0094] Example 3 In vivo efficacy evaluation of ginsenoside CK and bicyclol in the treatment of liver cancer
[0095] Establishment of human liver cancer xenograft tumor model in nude mice: female BALB / c nude mice aged 4-5 weeks were subcutaneously inoculated with Hep-3B cells in the left axilla, and each nude mouse was injected with 4×10 6 cells. Tumor volume reaches 100-300mm 3 , the nude mice were randomly divided into 16 groups, with 10 animals in each group.
[0096] The 16 groups were 1 model group and 15 drug-dosing groups, and the 15 drug-dosing groups were: (1) 5 ginsenoside CK groups, administered by intragastric administration, the dosages were 60mg / kg and 30mg / kg respectively . kg; (3) 5 ginsenoside CK / bicyclol combination groups, intragastric administration, the doses of ginsenoside CK / bicyclol were 60mg / kg / 60mg / kg, 30mg / kg / 90mg / kg, 90mg / kg / 30mg / kg, 100mg / kg / 20mg / kg, 24mg / kg / 96mg / kg. The administration volume of each dosing group was 0.2 mL, administered onc...
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