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A kind of method for extracting high-purity glucoraphanin
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A glucoraphanin, high-purity technology, applied in chemical instruments and methods, organic chemistry, sugar derivatives, etc., can solve the problems of high solvent consumption, cumbersome process, long operation cycle, etc., and achieve simple and easy process, solvent use The effect of small amount and easy operation
Active Publication Date: 2019-11-26
PIONEER HERB IND CO LTD
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Although the purity of glucoraphanin obtained in this patent is relatively high, it needs to be separated by macroporous adsorption resin and silica gel column, and it needs to be loaded on the column twice and eluted with solvent. The process is relatively cumbersome, the solvent consumption is large, and the operation cycle is relatively long
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Embodiment 1
[0025] (1) Take 200 g of dried broccoli seeds, treat them in an oven at 105°C for 30 minutes, then crush them to 20 mesh with a pulverizer, add 500 g of petroleum ether, heat to 60°C for extraction for 1 hour, and filter to obtain defatted broccoli seeds.
[0026] (2) Add 1000 g of deionized water to the defatted broccoli seeds, extract at 80°C for 2 hours, filter, repeat the extraction once, and combine the extracts.
[0027] (3) Concentrate the glucoraphanin extract in a rotary evaporator to 200 mL, add 200 mL of 95% ethanol for alcohol precipitation, stand still for 12 hours, and filter to obtain a filtrate.
[0028] (4) The filtrate was concentrated to 100 mL and spray-dried to obtain the glucoraphanin product with a purity of 76.3% and a yield of 3.4%.
Embodiment 2
[0030] (1) Take 200 g of dried broccoli seeds, treat them in an oven at 100°C for 50 minutes, then crush them to 20 mesh with a pulverizer, add 600g of petroleum ether, heat to 60°C for extraction for 1 hour, and filter to obtain degreased broccoli seeds.
[0031] (2) Add 1000 g of deionized water to the degreased broccoli seeds, extract at 80° C. for 2 hours, and filter to obtain an extract.
[0032] (3) Concentrate the glucoraphanin extract in a rotary evaporator to 150 mL, add 200 mL of 95% ethanol for alcohol precipitation, stand still for 24 hours, and filter to obtain a filtrate.
[0033] (4) The filtrate was concentrated to 100 mL and spray-dried to obtain the glucoraphanin product with a purity of 78.6% and a yield of 3.1%.
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Abstract
The invention relates to a method for extracting high-purity glucoraphanin. The method comprises the steps as follows: (1) performing enzyme deactivation on broccoli seeds; (2) crushing the broccoli seeds after enzyme deactivation; (3) degreasing the broccoli seeds through petroleum ether extraction; (4) performing aqueous extraction on the degreased broccoli seeds to obtain an aqueous extraction solution; (5) performing concentration, alcohol precipitation and filtration on the aqueous extraction solution to obtain filtrate; (6) performing concentration and spray drying on the filtrate to obtain the high-purity glucoraphanin product. Compared with the prior art, the extraction separation method is simple, adopts a simple and feasible process and is easy to industrialize, the whole process is low in cost and investment, the high-purity glucoraphanin product can be obtained (the purity is 75% or above), and the highest yield is 3.5%.
Description
technical field [0001] The invention relates to a method for purifying natural products, in particular to a method for extracting high-purity glucoraphanin. Background technique [0002] Glucoraphanin (4-methylsulfinylbutenyl glucosinolate) widely exists in cruciferous plants, such as broccoli, kale, Brussels sprouts, cauliflower, horseradish, Chinese cabbage, etc. [Chinese Agricultural Science Bulletin, 2008, 24 (2): 335-338; Food Science and Technology, 2009, 34 (12): 250-252], among which active ingredients are the most in broccoli and cauliflower, especially the highest content in broccoli seeds. The degradation products of glucoraphanin are mainly sulforaphane, sulforaphane nitrile, and oxazolidinethione, among which sulforaphane has the highest content [Food Science and Technology, 2009, 34(12): 250-252]. Sulforaphane is a type of isothiocyanate with the strongest anti-cancer activity found in vegetables so far, and it is currently recognized as one of the natural pro...
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