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Chemoprotectants from crucifer seeds and sprouts

A technology of cruciferous and plant seeds, applied in food science, anti-virus, drug combination, etc., can solve problems such as not guaranteeing success

Inactive Publication Date: 2007-03-28
INTERCONTINENTAL GREAT BRANDS LLC
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

See, for example, U.S. Patent Nos. 6,521,818, 6,242,018, 6,177,122, 5,968,567, 5,968,505, and 5,725,895; however, cultivating a germplasm from the laboratory to field trials and then to market will take a considerable amount of time, more than 5 years, and does not guaranteed success

Method used

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  • Chemoprotectants from crucifer seeds and sprouts

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

Example 1: Batch Processing of Broccoli Seeds

[0023] A 30 ml sample of the aqueous extract from 1 g of crushed defatted broccoli seeds (Gypsy variety) was treated with 100 mg of 12-20 mesh activated carbon (Dareo G-60) by boiling in 30 ml of water for 1 minute, followed by filtration to remove Spent sorbent, thereby providing an aqueous extract. The initial ratio of glucoraphanin / 4-hydroxyglucosinolate in the raw seeds was about 11, which increased to about 70 after the treatment. By high performance liquid chromatography (HPLC) West, L etc., J. Chromatogr. A 2002, 966, 227-232 measured that the loss of radish was about 4%.

Embodiment 2

Example 2: Column treatment of broccoli seeds

[0024] A 30 ml sample of the aqueous extract from 1 g of crushed defatted broccoli seeds (var. Gypsy) was fed into a column containing 1000 mg of graphitized carbon black. The glucoraphanin / 4-hydroxyglucosinolate ratio was initially about 11 in the original seed and rose to >1000. The loss of radish was about 13% as measured by HPLC.

Embodiment 3

Example 3: Boiling water treatment of broccoli seeds

[0025] 100 mg of activated charcoal (Darco G-60) was mixed with 1 g of crushed defatted broccoli seeds (Gypsy variety) and extracted in boiling water followed by centrifugation to remove particles. The initial ratio of glucoraphanin / 4-hydroxyglucosinolate was about 11, rising to about 30. The loss of radish was about 2% as measured by HPLC.

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Abstract

The present invention is directed to chemoprotectant precursor compositions provided from crucifer seeds and sprouts and methods for their preparation. Treatment of aqueous extracts from crucifer seeds or sprouts with adsorbents substantially increases the ratio of certain highly chemoprotectant precursor compounds (alkyl glucosinolates such as glucoraphanin, a.k.a. sulforaphane glucosinolate) to undesirable compounds such as indole glucosinolates (for example 4-hydroxyglucobrassicin). The method provides an extract which has a ratio of glucoraphanin to 4-hydroxyglucobrassicin of about 70 or greater. The resulting extract has improved color and odor and may be dried or used directly as an additive in a variety of foodstuffs.

Description

technical field [0001] The present invention relates to chemoprotective compounds and methods for preparing chemoprotective compounds, wherein said compounds can be incorporated into a variety of food products. More specifically, an extraction method is provided that efficiently provides an extract having a high ratio of chemoprotected compounds / less important compounds while also providing a high yield of chemoprotected compounds. Increased yields and ratios of chemoprotected compounds were achieved by combining the water extraction method used with adsorbents. Background technique [0002] It is widely recognized that diet plays a large role in controlling the risk of cancer development, and that increased consumption of fruits and vegetables may reduce cancer rates in humans. The presence of certain trace chemical constituents in plants provides the primary protective mechanism when delivering them to mammalian cells. [0003] Cruciferous plants contain phytochemical pr...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23L1/212A23L1/30A23L19/00
CPCA23L1/3002A23L33/105A61P3/02A61P35/00A61P39/06
Inventor L·G·维斯特G·W·哈斯N·马图舍斯基
Owner INTERCONTINENTAL GREAT BRANDS LLC
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