Method for breeding high glucoraphanin content Brassica rapa

Abstract

The invention relates to a method for breeding high glucoraphanin content Brassica rapa. The method comprises A: introducing a gene which is lost in function in a donor material into a receptor material to be cultured so that a polymerized single plant is obtained, and B: systematically breeding the polymerized single plant to obtain high glucoraphanin content Brassica rapa, wherein the gene losing functions comprises at least one of BrAOP2.1, BrAOP2.2 and BrAOP2.3. The high glucoraphanin content Brassica rapa contains the protein losing functions and the protein losing functions comprises at least two of BrAOP2.1, BrAOP2.2 and BrAOP2.3. The method utilizes a molecular marker-assisted selection technique, saves a breeding cost, shortens breeding time and improves breeding efficiency.

Description

technical field [0001] The invention belongs to the technical field of breeding, and in particular relates to a method for breeding cabbage crops with high content of 4-methylthiooxybutyl glucosinolate. Background technique [0002] Cabbage crops (Brassica rapa, L.syn.Brassica campestris) belong to Brassicaceae Brassica, including many important vegetables, feed and oil crops. my country is the origin and evolution center of cabbage crops. Cabbage crops have been cultivated in my country for more than 2,000 years, and have gradually evolved into various types including Chinese cabbage, pakchoi, turnip, cabbage, cabbage, etc., becoming the most widely distributed and planted vegetables in my country One of the most important crops, it plays an important role in agricultural production. [0003] Glucosinolate glucoside (glucosinolate for short) is a unique secondary metabolite in cruciferous plants. After the plant cells are destroyed, this metabolite immediately combines wit...

AI Technical Summary

Problems solved by technology

Although whole-genome duplication events have occurred in both cabbage and cabbage crops, and there are three AOP2 genes, all three AOP2 genes are active in cabbage crops, while two AOP2 genes in cabbage are due to base deletions. , causing premature termination, making it non-functional

This may be responsible for the higher GRA content of cabbage crops

In addition, due to the existence of AOP2 gene redundancy, the loss of function of one copy may not significantly affect the accumulation of the glucosinolate GRA in the body.

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