Method for preparing sulforaphene

Abstract

The invention discloses a method for preparing sulforaphene, and belongs to the technical field of separation and purification. The glucoraphanin hydrolysate is prepared according to the method disclosed by the patent with the patent number of 201510270292.5, and then a sulforaphene crude separation solution is prepared, a TBP or TRPO sulfonated kerosene extraction solution is prepared, a sulforaphene primary purification solution is prepared, a purified sulforaphene concentrated solution is prepared and finally the sulforaphene is prepared, wherein the purity of the sulforaphene product is 99.14%. The method is simple and convenient to operate, accurate in separation, mild in condition, energy-saving and low in production cost, the extraction agent can be recycled, the product is stable, the purity is high, the byproducts generated in the production process are effectively utilized, no 'three wastes' is generated, and the method is a typical green production process, so that the problems that the sulforaphene is easily rearranged or isomerized in water, and the product is not easy to store for a long time are solved. The sulforaphane product prepared by the method can be widely applied to the fields of health products and medicines.

Description

[0001] 1. Technical field [0002] The invention belongs to the technical field of separation and purification, and in particular relates to a method for preparing radishin. [0003] 2. Background technology [0004] Cruciferous plants contain glucosinolates (referred to as glucosinolates). Modern research has found that under the catalysis of glucosinosidase (myrosinase), glucosinolates are hydrolyzed and rearranged to generate cyanate, isothio Compounds such as cyanates, nitriles or oxazolidines. Sulforaphane is a kind of isothiocyanate, which has anti-tumor, detoxification, antibacterial, and anti-oxidation effects. It has obvious blocking effects on liver cancer, breast cancer, lung cancer, esophageal cancer, and pregastric cancer. Natural active substances with high potential and economic value. [0005] Existing methods for separating sulforaphane include: a paper published in the 7th issue of volume 37 of "Food and Fermentation Engineering" in 2011, titled "Separation ...

AI Technical Summary

Problems solved by technology

The main problem that above-mentioned two kinds of methods exist is: 1. select organic solvents such as dichloromethane, ethyl acetate to extract sulforaphane, this type of solvent is volatile or highly water-soluble, is difficult to recover completely, pollutes environment; 2. selectivity is low Macroporous adsorption resin adsorbs the target, it is difficult to obtain specific adsorption, and the adsorption and separation time is as long as several hours, while sulforaphane begins to rearrange or isomerize impurities such as thiocyanate or isoxane in aqueous solution within 30 minutes , therefore, subsequent separation and purification are difficult and inefficient, and it is difficult to obtain high-purity sulforaphane