Method for detecting oxidized form glucoraphanin and reduced form glucoraphanin from radish extract

Abstract

The invention discloses a method for detecting oxidized form glucoraphanin and reduced form glucoraphanin from a radish extract. The method comprises the following steps: dissolving an oxidized form glucoraphanin and reduced form glucoraphanin standard product into a methanol-aqueous solution, and diluting to obtain a standard product solution with series mass concentration; dissolving the to-be-tested radish extract into a methanol-aqueous solution to obtain a radish extract sample solution; performing high performance liquid chromatography analysis on the standard product solution with series mass concentration and the radish extract sample solution separately to obtain each chromatographic peak area; establishing standard curve equations of the oxidized form glucoraphanin and the reduced form glucoraphanin separately; and substituting the chromatographic peak area of the oxidized form glucoraphanin and the chromatographic peak area of the reduced form glucoraphanin in the sample solution into the corresponding curve equations to obtain the mass concentration of the two types of glucoraphanin in the sample solution, and calculating to obtain the oxidized form glucoraphanin content and the reduced form glucoraphanin content of the radish extract. The method realizes synchronous and quantitative detection of the oxidized form glucoraphanin and the reduced form glucoraphanin, and has the advantages of high speed, high efficiency and high accuracy.

Description

technical field [0001] The invention belongs to the technical field of quantitative detection of plant active components, and in particular relates to a simultaneous quantitative detection method for oxidized and reduced glucoraphanin in radish extracts. Background technique [0002] A large number of studies have shown that the natural active ingredients contained in cruciferous vegetables have the effect of preventing various cancers. The probability of various cancers among people who often eat a lot of cruciferous vegetables is significantly lower than that of those who do not eat or eat a small amount of cruciferous vegetables crowd. Cruciferous vegetables contain a large amount of glucosinolate and myrosinase. During the processing and consumption of cruciferous vegetables, myrosinase can rapidly hydrolyze glucosinolate and convert it into isoglucosinolate with high anticancer activity. Thiocyanate, which promotes the anti-cancer and anti-cancer effects of cruciferous...

AI Technical Summary

Problems solved by technology

However, the stability of oxidized and reduced sulforaphane is extremely poor, easy to degrade, and they are easy to react with sulfhydryl-containing compounds to lose their anticancer activity, while their precursors, oxidized and reduced sulforaphane, are chemically stable , not easy to degrade, and after being ingested by the human body, it can be transformed into oxidized and reduced sulforaphane with high anticancer activity under the action of intestinal microorganisms. Therefore, both oxidized and reduced sulforaphane can be used as Anti-cancer and anti-cancer natural active ingredients, extract radish extract rich in oxidized and reduced glucoraphanin from radish, and add it to food, functional food, health care products and medicines, it will better promote The Development of Cancer Prevention and Treatment in China

[0004] At present, the existing detection methods of oxidized and reduced glucoraphanin are mainly high-performance liquid chromatography, which is an effective method for detecting the content of oxidized and reduced glucoraphanin, with high accuracy and precision However, the existing detection methods are all quantitative detection of oxidized glucoraphanin or reduced glucoraphanin alone, and further improvement is needed in terms of mobile phase selection, detection wavelength selection, detection accuracy and precision, etc.

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