Test strip for simultaneously and quantitatively detecting NSE and S100B in blood and preparation method thereof

A quantitative detection and test strip technology, which is applied in the field of medical detection, can solve the problems of inability to realize ultra-early diagnosis, high professional level requirements, and expensive detection instruments, so as to achieve good clinical application significance, improve accuracy and sensitivity, and high sensitivity Effect

Pending Publication Date: 2019-11-19
SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Neuroimaging techniques (CT, MRI, TCD and PET) are the most widely used clinical diagnostic methods, which can effectively detect and distinguish between ischemic stroke and hemorrhagic stroke, but there are still some deficiencies and deficiencies, such as Diffe

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  • Test strip for simultaneously and quantitatively detecting NSE and S100B in blood and preparation method thereof

Examples

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Example Embodiment

[0029] Embodiment 1. This embodiment provides a magnetic immunochromatographic test strip for simultaneous quantitative detection of NSE and S100B in blood and a preparation method thereof.

[0030] The NSE paired antibody and S100B paired antibody used in this example are both monoclonal antibodies prepared by monoclonal antibody technology. Use the principle of double-antibody sandwich detection of NSE and S100B antigens to detect specimens. When the specimen to be tested contains NSE and S100 antigens, the antigens will first be combined with the magnetic beads coupled to the NSE antibody and the magnetic beads coupled to the S100B antibody on the binding pad. As the chromatography progresses, the conjugate moves forward to reach the S100B antibody detection line T2, the S100B antigen will combine with the coated antibody again to form a double antibody sandwich complex and gather at T2, and the conjugate moves forward to reach the NSE antibody At the detection line T1, the NS...

Example Embodiment

[0052] Example 2

[0053] Except for the preparation steps of immunomagnetic beads: the addition amount of EDC and NHS are both 5 times the molar amount of the carboxylation degree of the magnetic beads, the other steps are the same as in Example 1.

Example Embodiment

[0054] Example 3

[0055] Except for the bonding pad preparation step, the content of sucrose in the bonding pad treatment solution used is 10 wt%, and the other steps are the same as in Example 1.

[0056] Method of using the detection card of the present invention

[0057] 1. Add samples

[0058] Take out the single test card from the packaging box, tear open the aluminum foil packaging bag, place it on a flat table, and use a micropipette to draw 70 μL of the serum sample into the sample hole of the test card, and wait for the reaction to proceed for 20 minutes.

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Abstract

The invention belongs to the technical field of medical detection, and relates to a magnetic immunochromatographic test strip for simultaneously and quantitatively detecting NSE and S100B in blood anda preparation method thereof. The test strip is improved, and a magnetic immunochromatographic technology is creatively introduced into the combined detection of the NSE and the S100B for the first time, and by combining a magnetic signal detector, quantitative detection of NSE and the S100B for a single person can be realized simultaneously; and the test strip has the characteristics of being rapid, simple, practical, high in specificity and high in sensitivity, and can be used for remarkably improving the accuracy of cerebral apoplexy detection, that is, on the basis of double positive of NSE and S100B, the cerebral apoplexy occurrence rate can be further judged, so that the early diagnosis rate and prognosis monitoring of cerebral apoplexy can be improved, and the method has relativelyhigh clinical application significance.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and relates to a magnetic immunochromatographic test strip and a preparation method for quantitatively detecting NSE and S100B in blood simultaneously. Background technique [0002] Stroke (stroke) is one of the three major causes of death for human beings, and the number of patients is increasing year by year. It has the characteristics of high fatality rate, high disability rate and high recurrence rate, which seriously endangers people's health and life safety. , financial and social have a huge burden. Ischemic stroke accounts for 60-80% of strokes, and the number of patients with hemorrhagic stroke is lower than that of ischemic stroke patients, but it has a higher disability rate and worse prognostic effect. Therefore, rapid and accurate early diagnosis is helpful to formulate treatment measures and reduce the mortality and disability rate of stroke. Neuroimaging techniques (CT...

Claims

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Application Information

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IPC IPC(8): G01N33/558G01N33/531G01N33/543
CPCG01N33/558G01N33/531G01N33/54326
Inventor 王莹杨明峰孙保亮范存东张宗勇袁慧
Owner SHANDONG FIRST MEDICAL UNIV & SHANDONG ACADEMY OF MEDICAL SCI
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