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Method for preparing glucoraphanin from glucoerucin

A technology of arugulaside and sulforaphane, which is applied in the field of biochemical pharmacy, can solve the problems of excessive use of reagents, complicated operation, cost and energy consumption, etc., and achieves the effects of good large-scale industrial application, simple operation and low energy consumption.

Inactive Publication Date: 2019-06-21
山东益康药业股份有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] Aiming at the deficiencies of the above-mentioned prior art, the present invention provides a method for preparing sulforaphane based on arugin, the preparation method of the present invention overcomes the need to use more reagents in the process of preparing sulforaphane from traditional arugin, and has Solvent residue, cumbersome operation, complicated process, and after passing through the column, due to the disadvantages of cost and energy loss caused by the existence of KCl and the clogging of the preparation chromatographic column, it provides high-purity raw materials for the preparation of sulforaphane, so it has a good practical The value of application and the prospect of large-scale industrial application

Method used

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  • Method for preparing glucoraphanin from glucoerucin
  • Method for preparing glucoraphanin from glucoerucin
  • Method for preparing glucoraphanin from glucoerucin

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preparation example Construction

[0030] Preparation of Glucose Sulforaphane (GRA): Glucose sulforaphane is obtained by oxidizing solid aruginoside GER.

[0031] In another specific embodiment of the present invention, in the preparation process of the arugin (GER), the high-temperature stir-frying treatment conditions are specifically: stir-frying at 110-130° C. (preferably 120° C.) for 15-30 min (preferably 20 min) If the temperature is too low, it is not conducive to the subsequent degreasing, and if the temperature is too high, the active ingredients such as arugin will be inactivated.

[0032] In another specific embodiment of the present invention, the specific method of degreasing is: adding petroleum ether to the pulverized arugula seeds, stirring, soaking and degreasing, and the material-to-liquid ratio of the pulverized arugula seeds to the petroleum ether is 1 g: 1.5-3 mL (preferably 1g: 2mL);

[0033] In yet another specific embodiment of the present invention, the specific method of the thermal e...

Embodiment 1

[0047] (1) Preparation of arugin (GER)

[0048] Stir fry 100 g of arugula seeds at 120° C. for 20 minutes, and then pulverize after cooling. 200 mL of petroleum ether was added for stirring, soaking and degreasing, and the supernatant was removed by centrifugation. The solid powder was naturally air-dried at room temperature. Take the air-dried solid powder, add 1 L of deionized water, extract with boiling water at 120°C for 20 minutes, suction filter and collect the filtrate. The filter cake was extracted twice, and the two filtrates were combined to obtain a crude extract of GER.

[0049] Two resin columns in series are used for separation and purification from crude extract, the first resin column is filled with polyglycidyl methacrylate (PGMA) resin for decolorization of crude extract, and the second resin column is filled with strong base Anion exchange resin D261 resin is used for adsorption separation. After the adsorption reached saturation, 10% ammonia water was se...

Embodiment 2

[0057] (1) Preparation of arugin (GER)

[0058] Stir fry 100 g of arugula seeds at 120° C. for 20 minutes, and then pulverize after cooling. Add 250 ml of petroleum ether for stirring, soaking and degreasing, centrifuging to remove the supernatant, and the solid powder is naturally air-dried at room temperature. The air-dried solid powder was added to 1.1 L of deionized water, extracted with boiling water at 120° C. for 20 minutes, and the filtrate was collected by suction filtration. The filter cake was extracted twice, and the two filtrates were combined to obtain a crude extract of GER.

[0059] Two resin columns in series are used for separation and purification from crude extract, the first resin column is filled with polyglycidyl methacrylate (PGMA) resin for decolorization of crude extract, and the second resin column is filled with strong base Anion exchange resin D261 resin is used for adsorption separation. After the adsorption reached saturation, 10% ammonia wate...

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Abstract

The invention provides a method for preparing glucoraphanin from glucoerucin and belongs to the technical field of biochemical pharmacy. The method overcomes the defects of high reagent dosage, residual solvents, complicated operation and technologies in a traditional process of preparing the glucoraphanin from the glucoerucin as well as high cost, energy loss and chromatographic column blockage in the presence of KCl after column chromatography isolation, thereby being high in practical application value and promising in large-scale industrial application prospect.

Description

technical field [0001] The invention belongs to the technical field of biochemical pharmacy, and in particular relates to a method for preparing glucose sulforaphane based on arugulin. Background technique [0002] The information disclosed in this Background section is only for enhancement of understanding of the general background of the invention and should not necessarily be taken as an acknowledgement or any form of suggestion that this information forms the prior art already known to a person of ordinary skill in the art. [0003] Glucosinolates (GLS) are widely found in cruciferous plants. Arugula glycosides (glucoerucin, GER) is a kind of glucosinolates, accounting for more than 95% of the total glucosinolates in arugula, can be oxidized into glucoraphanin (glucoraphanin, GRA). Glucose sulforaphane is the precursor of sulforaphane (SF), which can be obtained by hydrolysis of glucose sulforaphane by myrosinase. The reaction principle is as follows: [0004] [0...

Claims

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Application Information

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IPC IPC(8): C07H15/14C07H1/00C07H1/06
Inventor 梁浩高肇林王付苍张兴柱史倩文魏正凤
Owner 山东益康药业股份有限公司
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