Method for preparing glucoraphanin from broccoli

A technology of glucoraphanin and cauliflower, which is applied to the preparation of sugar derivatives, chemical instruments and methods, sugar derivatives, etc., and can solve the problems of difficult industrial production and high cost of raw material selection

Active Publication Date: 2014-04-09
HEILONGJIANG DINGHENGSHENG PHARM CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The preparation process of glucoraphanin in the above-mentioned patent documents is mainly extracted through steps such as hot water extraction and chromatographic column separation. Although the final glucoraphanin has a high purity, the process requires too mu

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0026] Weigh 2T of cauliflower raw material, chop it after cleaning, and add it at a constant speed of 400kg / h from the feeding port of the elevator, and add 75% ethanol aqueous solution through the liquid inlet pipe at a flow rate of 2T / h with a pipeline pump, and the frequency of the elevator is set to 30Hz, host 1 frequency is set to 20Hz, juicer 1 frequency is set to 50Hz, host 2 frequency is set to 22Hz, juicer 2 frequency is set to 45Hz, and glucoraphanin crude extract 10T, glucoraphanin The crude extract flows to the temporary storage tank by itself and is continuously pumped to the concentration tank by the outlet pump to concentrate to obtain 600L of crude glucoraphanin concentrate; dilute the obtained concentrate with 3T water and filter to remove impurities; the filtrate is passed through HPD400 macroporous adsorption resin, resin The ratio of column height to diameter is 5:1. After adsorption is saturated, impurities are washed with 6T water, 2T with 5% ethanol aque...

Embodiment 2

[0028] Weigh 800kg of cauliflower raw material, chop it after cleaning, add it at a constant speed of 200kg / h from the feeding port of the elevator, and add 65% ethanol water solution through the liquid inlet pipe at a flow rate of 1T / h with a pipeline pump, and set the frequency of the elevator as 25Hz, host 1 frequency is set to 18Hz, juicer 1 frequency is set to 50Hz, host 2 frequency is set to 22Hz, juicer 2 frequency is set to 45Hz, and glucoraphanin crude extract 4T is obtained; glucoraphanin The crude extract flows to the temporary storage tank by itself and is continuously pumped to the concentration tank by the discharge pump to concentrate to obtain 250L crude glucoraphanin concentrate; dilute the obtained concentrate with 1.25T water, filter to remove impurities; the filtrate is absorbed through AB-8 macropores Resin, the ratio of height to diameter of the resin column is 5:1, after the adsorption is saturated, wash impurities with 6T water, wash impurities with 20% ...

Embodiment 3

[0030] Weigh 1.6T of cauliflower raw material, chop it after cleaning, add it at a constant speed of 100kg / h from the feeding port of the elevator, add 75% ethanol water solution through the liquid inlet pipe at a flow rate of 500kg / h with a pipeline pump, and set the frequency of the elevator 25Hz, host 1 frequency is set to 18Hz, juicer 1 frequency is set to 50Hz, host 2 frequency is set to 22Hz, juicer 2 frequency is set to 45Hz, glucoraphanin crude extract 8T; sulforaphane The crude glucoraphanin extract flows automatically to the temporary storage tank and is continuously pumped by the outlet pump to the concentration tank to concentrate to obtain 500L of crude glucoraphanin concentrate; dilute the obtained concentrate with 2.5T water, filter to remove impurities; filter the filtrate through DM-1308T70% ethanol Elute with aqueous solution to obtain 7.8T of glucoraphanin eluent; concentrate the eluent to obtain 310 kg of glucoraphanin concentrated paste; mix 310 kg of activ...

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PUM

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Abstract

The invention discloses a method for preparing glucoraphanin from broccoli, which comprises the following steps: cleaning the raw material, chopping, adding into an extraction tank set host, and sufficiently contacting solid-phase and liquid-phase substances in the extraction tank set in a reverse motion process, thereby extracting effective components from the medicinal material. The medicine slag are forcedly pushed to a slag outlet to be discharged by a discharge conveyer, and a special squeezing machine extrudes the medicine slag to obtain medicinal material tissues from the residual liquor in the medicine slag, thereby reducing the residual liquor content in the medicine slag. The method has the advantages of higher extraction efficiency and more complete extraction. By adopting the macroporous adsorbent resin combined with silica gel column chromatography for purification, the separating effect is better. The method can be used for preparing the glucoraphanin finished product of which the maximum yield is 1% and the content is higher than 95%.

Description

Technical field: [0001] The invention belongs to the field of natural organic chemistry, and relates to a preparation method of glucoraphanin, in particular to a preparation method of separating and purifying glucoraphanin by using a macroporous adsorption resin coupled with silica gel column chromatography. Background technique: [0002] Cauliflower (scientific name: Brassica oleracea L.var.botrytis L.), also known as cauliflower, cauliflower or cauliflower, is a cruciferous vegetable, a variant of cabbage. [0003] Biennial herb, 60-90 cm high, covered with pink frost. Stem erect, stout, branched. Basal leaves and lower leaves are oblong to elliptic, 2-3.5 cm long, gray-green, rounded at the top, spreading, not convoluted, entire or with fine teeth, sometimes the leaves are decurrent, with several small lobes, And wing-shaped; petiole 2-3 cm long; middle and upper leaves of stem are small and sessile, oblong to lanceolate, clasping. There is a milky white fleshy head at...

Claims

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Application Information

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IPC IPC(8): C07H15/14C07H1/08
Inventor 姚德坤苏学辉姚德利张亚红姚航黄运生吴海龙孟祥敏
Owner HEILONGJIANG DINGHENGSHENG PHARM CO LTD
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