Short bifidobacteria with functions of anti-gastrointestinal tract pathogen, oxidation resistance and blood pressure reduction

A bifidobacterium breve, a technology for use, applied in the field of microbiology, can solve problems such as threats to human safety, insufficient probiotic effects, and insufficient inhibitory effect on pathogenic bacteria

Active Publication Date: 2008-12-03
PRESIDENT ENTERPRISES (CHINA) INVESTMENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Although a variety of bifidobacteria have been isolated in this field and these bifidobacteria have certain functions, some bifidobacteria are not isolated from the human body, and may cause certain safety threats to the human body after taking them; and most bifidobacteria The inhibitory effect of bacillus on pathogenic bacteria is not strong enough, and the probiotic effect is not obvious enough

Method used

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  • Short bifidobacteria with functions of anti-gastrointestinal tract pathogen, oxidation resistance and blood pressure reduction
  • Short bifidobacteria with functions of anti-gastrointestinal tract pathogen, oxidation resistance and blood pressure reduction
  • Short bifidobacteria with functions of anti-gastrointestinal tract pathogen, oxidation resistance and blood pressure reduction

Examples

Experimental program
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Effect test

Embodiment 1

[0084] Example 1: Source and Collection of Samples

[0085] The bacterial strain of the present invention is derived from feces of long-lived old people in Hotan area, Xinjiang, a longevity village in China.

[0086] Located at the southernmost tip of Xinjiang, Hotan was called Khotan in ancient times, which means "the place where jade is produced" in Tibetan. It is an important town on the southern route of the Silk Road. Hotan has a total area of ​​248,000 square kilometers and a population of 1.5 million. Among them, Uighurs account for more than 97% of the total population. As early as the Shang and Zhou dynasties, ancient Khotan had material exchanges with the Central Plains. In 68 BC, Emperor Xuan of the Han Dynasty "sent the guards to protect the countries west of Shan", and Khotan was formally brought under the jurisdiction of the central government of the Han Dynasty. Ancient Khotan was the earliest Buddhist center in the Western Regions, with a rich Buddhist cult...

Embodiment 2

[0088] Embodiment 2: Isolate lactic acid bacteria from feces of long-lived old people

[0089] The strain screening was carried out for 6 years (2000-2005). 26 feces samples of long-lived elderly people (over 90 years old) in Hetian, Xinjiang were immersed in MRS broth culture and incubated at 37°C for 24 hours. This culture was spread on an MRS agar plate, and then cultured at 37°C for 3 days. After cultivation, the colonies that appeared on the agar medium were collected and further purified. A total of 301 strains of acid-producing bacteria were obtained by colony morphology screening, and lactic acid bacteria were obtained by measuring their products. According to the growth characteristics and metabolic characteristics, a specific medium was selected , to obtain the dominant lactic acid bacteria. From the dominant lactic acid bacteria, according to its characteristics, a strain of lactic acid bacteria with excellent characteristics is obtained. The Bifidobacterium breve...

Embodiment 3

[0092] Example 3: Gram Stain and Culture Characterization

[0093] The characteristics of the strains were detected by Gram staining. The results of Gram staining were as figure 1 A and figure 1 As shown in B, the strain XJH301 is a gram-positive bacterium without spores, rod-shaped or branched, with a length of 1.0-1.5 μm, which is a typical feature of bifidobacteria.

[0094] Then, study the bacteria to elucidate its cultural characteristics. As a result, the strain grows into a cream-colored colony with a diameter of 1-2mm on the MRS agar plate, and the optimum culture temperature is 37°C. The genetic traits of the strain XJH301 are stable, and no variation in traits has been found after several generations of culture.

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Abstract

The invention discloses a novel Bifidobacterium breve with excellent probiotic function. The Bifidobacterium breve strains are obtained by screening the feces of the long-lived elders in Hetian, XinJiang, and the obtained strains have outstanding resistance to acid and bile acid salts, adhesion characteristics to intestinal tract epidermic cells, excellent antibacterial performance and good oxidization resistance; and the strains have an effect on reducing the blood pressure as well as the edible safety, therefore, the Bifidobacterium breve can be widely applied in the fields such as functional additives, dairy products, beverage, functional food, drug production, etc.

Description

technical field [0001] The present invention belongs to the field of microbiology, and more specifically, the present invention relates to a novel Bifidobacterium breve having the functions of resisting gastrointestinal pathogenic bacteria, anti-oxidation and lowering blood pressure, and a food composition comprising the strain or its metabolites substances or pharmaceutical compositions. Background technique [0002] Bifidobacterium is a Gram-positive bacterium, without spores, immobile, strictly anaerobic, and variable in shape. Different species or different ages of the same species, and different growth environments present various forms such as curved rods, V and Y shapes, etc. And hence the name Bifidobacterium. Bifidobacterium was first discovered and isolated in the feces of breastfed infants by Tisser of the Pasteur Institute in France in 1899, and further research found that in the human digestive tract, the number of bifidobacteria changes dynamically with age, a...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P9/14A61P1/00A61P31/04A61K35/745
Inventor 李正华顾瑞霞陈顺利余哲礼麻文博沈琴
Owner PRESIDENT ENTERPRISES (CHINA) INVESTMENT CO LTD
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