Method for extracting and refining docosahexaenoic acid from schizochytrium

A technology of docosahexaenoic acid and Schizochytrium, applied in the biological field, can solve the problem that fish oil resources are difficult to meet the increasing demand of DHA, and achieve the effects of reducing extraction cost and simple operation

Inactive Publication Date: 2010-02-03
XIAMEN KINGDOMWAY GROUP +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Deep-sea fish oil is the main raw material for extracting DHA, and the fish oil used for extracting DHA in the world is about 1.1×10 6 tons (Gunstone F, 2001,

Method used

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  • Method for extracting and refining docosahexaenoic acid from schizochytrium
  • Method for extracting and refining docosahexaenoic acid from schizochytrium
  • Method for extracting and refining docosahexaenoic acid from schizochytrium

Examples

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Effect test

Embodiment 1

[0024] Fatty acid extraction and refining of algae harvested by fermentation in 5L fermenter

[0025] Take a tube of preserved bacteria, inoculate it into a 250mL shake flask containing 50mL of culture medium, and culture it in a shaker at 26°C at a speed of 150rpm for 36h, then take 2mL of the bacteria liquid and inoculate it into the above-mentioned fresh culture medium. The same method is continuously cultivated for three generations to obtain a seed liquid with higher vigor (in the logarithmic growth phase); then inoculate the fermenter of this seed liquid to 5L with a 10% inoculum size (the fermentation medium loading liquid is generally 100% of the fermenter) 60%, i.e. 3L, the same below), carry out stirring culture, the stirring speed is between 100-200, the air volume is at 0.2-2vvm, and the dissolved oxygen in the fermenter is controlled between 24-100% by controlling the rotating speed (fermentation The dissolved oxygen in the tank was set at 1 (ie 100%) at the begin...

Embodiment 2

[0034] Fatty acid extraction and refining of algae harvested by fermentation in 5L fermenter

[0035] Take a tube of preserved bacteria, inoculate it into a 250mL shake flask containing 50mL of culture medium, and culture it in a shaker at 26°C at a speed of 150rpm for 36h, then take 2mL of the bacteria liquid and inoculate it into the above-mentioned fresh culture medium. Continuously cultivate three generations in the same way to obtain a seed solution with higher vigor (in the logarithmic growth phase); then inoculate the seed solution with 10% inoculum in a 5L fermenter for stirring and culturing, and the stirring speed is between 100-200 During the period, the air volume is 0.2-2vvm, and the dissolved oxygen in the fermenter is controlled between 24-100% by controlling the rotating speed (the dissolved oxygen when the fermenter starts to cultivate is set at 1, which is 100%), and the pH is maintained by automatically adding ammonia water Between 6-7. The sugar concentrat...

Embodiment 3

[0044] Fatty acid extraction and refining of algae harvested by fermentation in 30L fermenter

[0045] Take a tube of preserved bacteria, inoculate it into a 250mL shake flask containing 50mL of culture medium, and culture it in a shaker at 26°C at a speed of 150rpm for 36h, then take 2mL of the bacteria liquid and inoculate it into the above-mentioned fresh culture medium. Continuously cultivate three generations in the same way to obtain a seed solution with higher vigor (in the logarithmic growth phase); then inoculate the seed solution with 10% inoculum in a 30L fermenter for stirring and culturing, and the stirring speed is between 100-200 During the period, the air volume is 0.3-3vvm, and the dissolved oxygen in the fermenter is controlled between 20-100% by controlling the rotating speed (the dissolved oxygen when the fermenter starts to cultivate is set at 1, which is 100%), and the pH is maintained by automatically adding ammonia water Between 6-7. The sugar concentr...

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Abstract

The invention discloses a method for extracting and refining docosahexaenoic acid from schizochytrium, which is characterized by comprising the following steps: carrying out flocculation processing ofschizochytrium ATCC 20888 fermentation liquid, then carrying out solid-liquid separation, mechanically crushing cells after alkali cell wall is broken, and extracting crushed thallus by adopting organic solvents to obtain DHA rough oil; processing the DHA rough oil by adopting the steps of degumming, alkali refining, decoloring and deodorizing to obtain DHA essential oil. The invention is simplein operation, can reduce the extraction cost of the DHA oil, only adopts the organic solvents during the operating process, has no residues finally, improves the quality of the DHA oil after being refined, has the DHA content in the extract about 28-51 percent and is suitable for large-scale production of DHA.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting and refining Docosahexaenoic acid (DHA for short) from Schizochytrium ATCC 20888. technical background [0002] Docosahexaenoic acid (DHA for short) is the long-chain polyunsaturated fatty acids (Lc-PUFAs) with the longest carbon chain and the highest degree of unsaturation found so far. DHA is an important structural component of biological membranes in the body, plays an important role in infant neurodevelopment, retinal formation, and intellectual development, and is also an autoregulatory substance with strong physiological activities such as prostaglandins, cycloprostaglandins, and leukotrienes. It has significant effects on preventing cardiovascular diseases, anti-lipidemia, lowering blood pressure, and inhibiting tumor formation (Hadley KB, et al, 2009, World Rev Nutr Diet.99: 97-104; Hawthorne KM, et al, 2009 , J Am Diet Assoc. 109:708-712.). Due to th...

Claims

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Application Information

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IPC IPC(8): C07C57/03C07C51/42C12P7/64C12R1/645
Inventor 江斌吴松刚黄建忠
Owner XIAMEN KINGDOMWAY GROUP
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