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Method for producing docosahexenoic acid by fermenting schizochytrium

A technology of docosahexaenoic acid and Schizochytrium, applied in the direction of microorganism-based methods, biochemical equipment and methods, fermentation, etc., to achieve the effects of high DHA purity, high DHA yield, safe and reliable quality

Active Publication Date: 2011-09-14
湖北福星生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although its DHA production has been greatly improved compared with the previous research, it is required to greatly reduce its production cost and market price for industrialized large-scale production, so that it can be vigorously promoted and popularized by ordinary consumers. still not enough

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Embodiment 1. The influence of the substratum formulation of fortified trace elements on DHA content and productive rate

[0047] Medium formula a (g / 100ml medium)

[0048] project

[0049] Fortified trace element medium formula b (g / 100ml medium)

[0050] project

[0051] Dipotassium phosphate

[0052] According to the formulas of medium a and b above, prepare 30L medium in 50L tanks, control the pH at 6.5, sterilize at high temperature, and cool to 30°C for later use.

[0053] Put the activated Schizochytrium sp.ATCC20888 slant strains into a 500ml Erlenmeyer flask with 100ml of culture medium, culture on a shaker at 28°C for 48 hours, and the shaker speed is 150r / min to prepare Schizochytrium sp. Seed liquid, its cell density is about 15g / l.

[0054] Insert above-mentioned seed liquid into above-mentioned prepared and sterilized culture medium according to 10% inoculum amount, pass into the air of 0.5VVM, supplement the glucose solutio...

Embodiment 2

[0058] Embodiment 2. Select ethanol to increase the impact of fermented liquid dissolved oxygen on DHA content and productive rate

[0059] Select the formula of medium b in Example 1, prepare two tanks of medium x and y according to the method described in Example 1, and inoculate Schizochytrium sp.ATCC20888 according to the method therein for fermentation, x The two tanks and y are basically the same in terms of fermentation control, and only differ in dissolved oxygen control. When tank y was fermented to 60 hours, 0.5% ethanol by volume of fermentation broth was added to increase the dissolved oxygen level in the fermentation broth, while tank x was used as a control to supplement 0.5% sterilized water by volume of fermentation broth accordingly.

[0060] Ferment and cultivate for 95 hours until the reducing sugar concentration is 3% and the amino nitrogen concentration is 0.037%, then the fermentation is terminated. Harvest the thalline by centrifugation, wash the thallu...

Embodiment 3

[0064] Embodiment 3. Select the influence of citric acid to adjust the pH of fermented liquid on DHA content and productive rate

[0065] Select the medium b formula in Example 1, prepare two tanks of medium X and Y according to the method described in Example 1, and inoculate Schizochytrium sp. ATCC20888 according to the method therein for fermentation and culture, X The two tanks and Y are basically the same in terms of fermentation control, and only differ in the acidity regulator for pH control. When tank X was fermenting for 40 hours, citric acid with a volume of 0.2% of the fermentation broth was added, and the pH of the fermentation broth was controlled at 6.8-7.0, while tank Y added the same volume of sulfuric acid during this process.

[0066] Ferment and cultivate for 95 hours until the reducing sugar concentration is 3% and the amino nitrogen concentration is 0.037%, then the fermentation is terminated. Harvest the thalline by centrifugation, wash the thallus fully...

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Abstract

The invention relates to a method for producing triglyceride type docosahexenoic acid fat by industrially fermenting schizochytrium. The method comprises the following step: the docosahexenoic acid fat is prepared by fermenting the schizochytrium taken as the strain with the a culture medium with microelements; wherein the culture medium with the microelements comprises a carbon source, a nitrogen source, inorganic salt and microelements, and the microelements comprise at least one of biotin, chlorophyll, beta-carotene, vitaminB1, vitaminB6 and vitaminB12. The prepared docosahexenoic acid fat does not need to be further chemically modified and can be directly used by people needing DHA, including infants, pregnant women, breast feeding women or patients who suffer from diseases due to insufficient DHA and need to supplement DHA. The invention has the advantages of high DHA content, high purity, safe and reliable quality, low production cost, stable production and almost no EPA; and the environmental-friendly, safe and high-content triglyceride type DHA fat can be produced with low cost at a large scale.

Description

technical field [0001] The invention relates to a microbial fermentation production method, in particular to the fermentative production of triglyceride type docosahexaenoic acid by using Schizochytrium strains. Background technique [0002] Docosahexaenoic acid (cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid, referred to as DHA) is an omega 3 series of long-chain polyunsaturated fatty acids, and is an important structural substance of the brain and retina. It can regulate the function of the central nervous system, prevent and treat cardiovascular diseases, eliminate inflammation, and inhibit cancer. [0003] Commercial sources of DHA are currently mainly fish oils and microalgae. The output and DHA content of fish oil are unstable, and the extract is mixed with fishy smell, and the DHA-containing mixed oil extracted from fish oil also contains eicosapentaenoic acid (EPA). According to research, EPA has a positive effect on the development of infants and young children. ne...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12P7/64C12R1/645
Inventor 徐从英夏德才林绍朗吴艳荣解秀娟
Owner 湖北福星生物科技有限公司
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