Method for producing docosahexenoic acid by fermenting schizochytrium

Abstract

The invention relates to a method for producing triglyceride type docosahexenoic acid fat by industrially fermenting schizochytrium. The method comprises the following step: the docosahexenoic acid fat is prepared by fermenting the schizochytrium taken as the strain with the a culture medium with microelements; wherein the culture medium with the microelements comprises a carbon source, a nitrogen source, inorganic salt and microelements, and the microelements comprise at least one of biotin, chlorophyll, beta-carotene, vitaminB1, vitaminB6 and vitaminB12. The prepared docosahexenoic acid fat does not need to be further chemically modified and can be directly used by people needing DHA, including infants, pregnant women, breast feeding women or patients who suffer from diseases due to insufficient DHA and need to supplement DHA. The invention has the advantages of high DHA content, high purity, safe and reliable quality, low production cost, stable production and almost no EPA; and the environmental-friendly, safe and high-content triglyceride type DHA fat can be produced with low cost at a large scale.

Description

technical field

[0001] The invention relates to a microbial fermentation production method, in particular to the fermentative production of triglyceride type docosahexaenoic acid by using Schizochytrium strains. Background technique

[0002] Docosahexaenoic acid (cis-4, 7, 10, 13, 16, 19-docosahexaenoic acid, referred to as DHA) is an omega 3 series of long-chain polyunsaturated fatty acids, and is an important structural substance of the brain and retina. It can regulate the function of the central nervous system, prevent and treat cardiovascular diseases, eliminate inflammation, and inhibit cancer.

[0003] Commercial sources of DHA are currently mainly fish oils and microalgae. The output and DHA content of fish oil are unstable, and the extract is mixed with fishy smell, and the DHA-containing mixed oil extracted from fish oil also contains eicosapentaenoic acid (EPA). According to research, EPA has a positive effect on the development of infants and young children. ne...