Method for extracting grease from schizochytrium

A technology for extracting oil from Schizochytrium algae, which is applied in the biological field and can solve the problems of difficulty in ethanol recovery, complicated process operation, large oil refining consumption, etc.

Active Publication Date: 2013-03-13
ENN SCI & TECH DEV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are inevitably some problems in this process, such as: part of the neutral oil is inevitably saponified; waste water pollutes the environment; when recovering fatty acids from by-product saponins, complex processing steps (hydrolysis, distillation) are required; especially for When refining crude oil with high acid value, the oil refining consumption is large, and the economic effect is not good
Moreover, in the current demulsification and delamination, methods such as adding ethanol and sodium chloride are mostly used for demulsification and delamination. In practical applications, this process has high production costs, complicated process operations, long delamination time, unstable delamination effect, Difficulty in recovering ethanol and producing a large amount of waste brine

Method used

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  • Method for extracting grease from schizochytrium

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0062] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.

[0063] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O 0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water.

[0064] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...

Embodiment 2

[0074] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.

[0075] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O 0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water.

[0076] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...

Embodiment 3

[0086] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.

[0087] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O 0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water;

[0088] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...

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Abstract

The invention discloses a method for extracting grease from schizochytrium. The method comprises the following steps: (1) adjusting the pH (Potential of Hydrogen) of the fermentation broth of the schizochytrium until reaching an acid stage, and then adding an organic solvent to stir, so as to realize the wall breaking of schizochytrium cells and extraction of microalgae oil; (2) adding a flocculant to the fermentation broth obtained in step (1) to flocculate, and extracting by the organic solvent to obtain crude algae oil; and (3) treating the crude algae oil by degumming, discoloring, and deacidifying / deodorizing, so as to obtain the microalgae oil rich in DHA (Docosahexaenoic Acid). According to the method disclosed by the invention, autolysis wall-breaking technology is adopted, so that the technological process in wall breaking and extraction of the schizochytrium can be simplified, the operation time in wall breaking can be shortened, the investment on equipment is decreased, and the cost is lowered; and the biological flocculant flocculation technology is carried out, therefore, the demulsification technological process is simplified, the time for layering is shortened, the layering effect is improved, the load due to wastewater discharge and water treatment caused by the demulsification process can be avoided, and the cost of water treatment can be lowered.

Description

technical field [0001] The invention belongs to the field of biotechnology, and relates to a method for extracting and refining oil-rich microalgae oil from Schizochytrium algae. Background technique [0002] Docosahexaenoic acid (DHA for short) is the long-chain highly unsaturated fatty acid with the longest carbon chain and the highest degree of unsaturation found so far. DHA is an important structural component of biological membranes in the body. It plays an important role in infant neurodevelopment, retinal formation, and intellectual development. It has significant effects on the prevention of cardiovascular diseases, anti-blood lipids, lowering blood pressure, and inhibiting tumor formation. Due to the above-mentioned multiple important physiological functions, DHA has become a fatty acid product with high value and wide market. [0003] Schizochytrium, also known as Schizochytrium, belongs to a class of marine fungi belonging to Eumycota, Oomycetes, Saprolegniales,...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C11B1/00C11B1/04C11B1/10C12P7/64C12R1/89
Inventor 杜彦山张雨忠刘敏胜徐春保
Owner ENN SCI & TECH DEV
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