Method for extracting grease from schizochytrium
A technology for extracting oil from Schizochytrium algae, which is applied in the biological field and can solve the problems of difficulty in ethanol recovery, complicated process operation, large oil refining consumption, etc.
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Embodiment 1
[0062] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.
[0063] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O 0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water.
[0064] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...
Embodiment 2
[0074] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.
[0075] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O 0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water.
[0076] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...
Embodiment 3
[0086] After the Schizochytrium ampoules were used to inoculate the slant, the liquid shake flask culture was carried out, and then transferred to the 6m 3 Fermentation tank culture, its medium composition is: glucose 100-130g, yeast extract 15-30g, peptone 10-20g, potassium dihydrogen phosphate 2-4g, magnesium sulfate 1-3g, trace element compound solution 8-11ml, 4-8ml of vitamin complex solution, dilute to 1L with 0.5×seawater; the 0.5×seawater is obtained by mixing 1 volume of seawater with 1 volume of water.
[0087] The preparation method of described trace element composite liquid is as follows: Na2·EDTA 6.0g, FeCl 3 ·6H 2 O 0.29g, H 3 BO 3 6.84g, MnCl 2 4H 2 O 0.86g, ZnCl 2 0.06g, CoCl 2 ·6H 2 O 0.026g, NiSO 4 ·6H 2 O0.052g, CuSO 4 ·5H 2 O 0.002g, NaMoO 4 2H 2 O 0.005g, dilute to 1L with water;
[0088] The preparation method of the vitamin complex solution is as follows: 100 mg of vitamin B1, 0.5 mg of vitamin B7, and 0.5 mg of vitamin B12 are diluted ...
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