Aurantiochytrium sp. YLH70 and application of Aurantiochytrium sp. YLH70 for synthesizing DHA

A technology of Schizochytrium and Jerusalem artichoke, applied in fungi, microorganism-based methods, microorganisms, etc., to achieve the effects of large yield, reduced production cost and low price

Inactive Publication Date: 2015-04-08
ZHEJIANG UNIV OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Based on the many advantages of fructose syrup, the method of using it to produce high-value products (such as DHA) has not been reported

Method used

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  • Aurantiochytrium sp. YLH70 and application of Aurantiochytrium sp. YLH70 for synthesizing DHA
  • Aurantiochytrium sp. YLH70 and application of Aurantiochytrium sp. YLH70 for synthesizing DHA
  • Aurantiochytrium sp. YLH70 and application of Aurantiochytrium sp. YLH70 for synthesizing DHA

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Embodiment 1

[0023] Example 1 Isolation and Identification of Schizochytrium YLH70

[0024] (1) Isolation of strain YLH70

[0025] Collect rotten leaf samples at the seaside of Yueqing Bay, Wenzhou City, Zhejiang Province, wash and put in YP culture solution (1g / L yeast powder, 1g / L peptone, 50mg / L ampicillin, 50mg / L streptomycin) suspended on the surface with 0.1g pine pollen prepared in water, pH 6.0), cultured in the dark at 30°C for 1 week, took 50 μL of the culture and spread it on a YP plate (1g / L yeast powder, 1g / L peptone, 50mg / L ampicillin, 50mg / L streptomycin Agar 20g / L, water preparation, pH value 6.0), cultured at 28°C for 3 days until a single colony grew out, repeated streaking and purification culture three times to obtain a pure bacterial strain, which was designated as bacterial strain YLH70.

[0026] (2) Identification of strain YLH70

[0027] The strain YLH70 was inoculated on a YP plate, cultured at 28°C for 2 days, the colony was yellowish white, round, moist and smo...

Embodiment 2

[0029] The preparation of embodiment 2 Jerusalem artichoke hydrolyzate

[0030] About 5000g of fresh Jerusalem artichoke was washed, sliced, and placed in an oven at 80°C for 24h to dry. The dried Jerusalem artichoke chips are pulverized by a solid pulverizer, and filtered through a 60-mesh sieve to obtain Jerusalem artichoke powder. Weigh 1000g of Jerusalem artichoke powder, mix it with 3000mL of 3% aqueous sulfuric acid solution, acidify at 80°C for 240min, filter to obtain Jerusalem artichoke juice (i.e. filtrate), adjust the pH to 6.0 with 1M NaOH aqueous solution. Add inulinase 40U / g Jerusalem artichoke powder, pectinase 2U / g Jerusalem artichoke powder, cellulase 10U / g Jerusalem artichoke powder to the filtrate, hydrolyze at a temperature of 50°C for 24 hours, filter to make Jerusalem artichoke hydrolyzate, and its reducing sugar concentration It is 145g / L, which is Jerusalem artichoke hydrolyzate.

Embodiment 3

[0031] The preparation of embodiment 3 Jerusalem artichoke hydrolyzate

[0032] Wash about 5000g of fresh Jerusalem artichoke, slice it, and place it in an oven at 40°C for 48h to dry. The dried Jerusalem artichoke chips are pulverized by a solid pulverizer, and filtered through a 60-mesh sieve to obtain Jerusalem artichoke powder. Weigh 3000g of Jerusalem artichoke powder, mix it with 3000mL of 10% sulfuric acid aqueous solution, acidify at 100°C for 30min, filter to obtain Jerusalem artichoke juice, and adjust the pH to 6.0 with 1M NaOH aqueous solution. Add inulinase 5U / g Jerusalem artichoke powder, pectinase 0.5U / g Jerusalem artichoke powder, cellulase 10U / g Jerusalem artichoke powder to the Jerusalem artichoke juice, hydrolyze it at a temperature of 30°C for 48 hours, filter to make a Jerusalem artichoke hydrolyzate, and reduce The sugar concentration was 256g / L.

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Abstract

The invention discloses a novel strain Aurantiochytrium sp. YLH70 and an application Aurantiochytrium sp. YLH70 for synthesizing DHA. The raw materials which are non-grain resource Jerusalem artichoke and plant starch sourced high fructose corn syrup are great in output and low in price, so that the production cost of DHA can be lowered; Jerusalem artichoke hydrolysate and high fructose corn syrup contain high concentration reducing sugar which is mainly sugars such as fructose, fructo-oligose and glucose which are easy to ferment, becomes a potential carbon source for producing DHA from Aurantiochytrium sp.; the fermenting biomass of the Aurantiochytrium sp. YLH70 strain with an excellent fermenting performance can reach 54.61-76.52g / L in a culture medium which takes the Jerusalem artichoke hydrolysate as a unique carbon source and the DHA output can reach 12.56-18-78g / L; in a culture medium which takes high fructose corn syrup as a unique carbon source, the fermenting biomass can reach 70.51-79.85g / L and the DHA output can reach 14.1-20.1g / L.

Description

(1) Technical field [0001] The invention relates to a marine protozoan Aurantiochytrium sp. YLH70 strain and a method for producing docosahexaenoic acid (DHA) by using Jerusalem artichoke hydrolyzate or fructose syrup. (2) Background technology [0002] DHA (Docosahexaenoic acid, Docosahexaenoic acid), known as "brain gold", is an indispensable important substance for the development of nervous systems such as vision and brain of adults and infants; it has the functions of preventing cardiovascular disease, anticancer, anti Physiological effects such as inflammation; adding a certain proportion of DHA to the bait can significantly improve the quality and growth rate of aquatic products and poultry. DHA is widely used in the fields of medicine, food and feed, and its broad market prospect has attracted great attention. The traditional DHA resource is fish oil. However, fish oil is currently facing problems such as resource scarcity, pollution, complicated process, poor taste...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/645
CPCC12P7/6427C12N1/145C12R2001/645
Inventor 于欣君汪钊郑建永章银军
Owner ZHEJIANG UNIV OF TECH
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