Aurantiochytrium sp. and application

A technology of Schizochytrium and strains, applied in Schizochytrium and its application field, can solve the problem of low DHA production, achieve the effects of short fermentation time, fast growth speed, and good industrial application value

Active Publication Date: 2014-09-10
QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the main bottleneck restricting its further promotion at present is the low output of DHA on the unit cost

Method used

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  • Aurantiochytrium sp. and application
  • Aurantiochytrium sp. and application
  • Aurantiochytrium sp. and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Schizochytrium, whose taxonomic name is Schizochytrium (Aurantiochytrium sp) SD006, has been preserved on October 21, 2013 at the China Microorganism Culture Collection Management Committee located at No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing General Microbiology Center (CGMCC), its deposit number is CGMCCNo: 8383.

[0022] The Schizochytrium strain is isolated from rotten leaves collected in mangrove waters in Zhanjiang City, Guangdong Province by a pine pollen fishing method. Observed under an optical microscope: the bacteria are spherical or ellipsoidal, with a diameter of 10-20 microns, and they mainly reproduce by division. With lateral unequal double flagella (see figure 1 ). Intracellular fatty acids are mainly composed of myristic acid, hexadecanoic acid, docosapentaenoic acid (DPA) and docosahexaenoic acid (DHA), and the content of DHA accounts for more than 55% of the total fatty acids. By comparing its 18S rDNA sequence with molecular bio...

Embodiment 2

[0062] Put the bacterial strain stored in the glycerol tube into a 250ml shake flask containing 50ml of seed medium, and culture it for 24 hours in a shaker at 35°C at a speed of 200rpm to obtain first-grade seeds; put the first-grade seeds into In a 500ml shake flask containing 100ml of seed medium, culture in a shaker at 35°C at a speed of 200rpm for 12 hours to obtain secondary seeds; add 1.2L of fermentation medium to a 3L bioreactor and insert activated secondary seed liquid. During the fermentation process, the temperature was controlled at 35°C. During the fermentation process, a nitrogen supplement operation is adopted, and 50% (w / v) corn steep liquor solution is fed within 24-48 hours of fermentation. The pH was maintained at 6.5 by automatic addition of 2M NaOH or 14% citric acid. The fermentation time is 60 hours.

[0063] After the fermentation, the yield of Schizochytrium obtained from the 3L fermenter was 190.25g / L, the yield of bio-oil was 104.6g / L, and the y...

Embodiment 3

[0067] Put the bacterial strain stored in the glycerol tube into a 250ml shake flask containing 50ml of seed medium, and cultivate it for 24 hours in a shaker at 32°C at a speed of 200rpm to obtain first-grade seeds; put the first-grade seeds into In a 500ml shake flask containing 100ml of seed medium, cultivate in a shaker at 32°C at a speed of 200rpm for 12 hours to obtain secondary seeds; add 3L of fermentation medium to a 5L bioreactor and insert the activated secondary seeds liquid. During the fermentation process, the temperature was controlled at 32°C. During the fermentation process, a nitrogen supplement operation is adopted, and 50% (w / v) corn steep liquor solution is fed within 24-48 hours of fermentation. The pH was maintained at 6.5 by automatic addition of 2M NaOH or 14% citric acid. The fermentation time is 70 hours.

[0068] After the fermentation, the yield of Schizochytrium obtained from the 5L fermenter was 202.86g / L, the yield of bio-oil was 114.1g / L, an...

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Abstract

The invention relates to the field of microbial fermentation engineering, and concretely relates to aurantiochytrium sp. and application. Aurantiochytrium sp. has the classification name of aurantiochytrium sp. SD006, is preserved in China General Microbiological Culture Collection Center, at No. 3, No. 1 yard, Beichen West Road, Chaoyang District, Beijing City since 21st, October, 2013, and the preservation number is CGMCC NO. 8383. Aurantiochytrium sp. is applicable to prepare docosahexenoic acid. The obtained bacterial strain has fast growth speed and short fermentation time. Docosahexenoic acid can be produced by utilizing high-density fermentation of aurantiochytrium sp. strains. Under the fermentation conditions shown in the specification, a maximum yield can be obtained in 60-70 h. After fermentation is performed for 70 h, the biomass obtained is 180-200 g/L, and the DHA output is 50-65 g/L.

Description

technical field [0001] The invention relates to the field of microbial fermentation engineering, in particular to a Schizochytrium and application thereof. Background technique [0002] Docosahexaenoic Acid (DHA) is an essential polyunsaturated fatty acid that must be supplemented from the diet. DHA is an important structural lipid component of the central nervous system and retina. It has the functions of strengthening the brain, improving memory and vision, especially promoting the development and growth of brain cells in fetuses and infants, improving the memory of adolescents, and preventing and treating senile dementia. Traditionally, DHA is mainly derived from deep-sea fish oil, but due to the great impact of overfishing, environmental pollution, seasonal changes, etc., it is gradually replaced by microbial fermentation production. At the present stage, the most important bacterial species for industrialized fermentation production of DHA is a marine microorganism—Aur...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12P7/64C12R1/645
Inventor 崔球宋晓金谭延振冯银刚
Owner QINGDAO INST OF BIOENERGY & BIOPROCESS TECH CHINESE ACADEMY OF SCI
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