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Algae with astaxanthin production and astaxanthin production by yeast mixed culture fermentation

A technology of mixed culture and astaxanthin, which is applied in fermentation and other directions, can solve the problems of astaxanthin accumulation affecting cell growth and low astaxanthin production, and achieve the effect of low cost and improving the conversion rate of sugar

Inactive Publication Date: 2003-09-03
TIANJIN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The current method of producing astaxanthin is to culture or ferment algae or yeast separately. During the respective culture or fermentation process, due to nutritional conditions, pH and CO 2 and O 2 The change of concentration affects the growth of cells and the accumulation of astaxanthin, and the production of astaxanthin is low

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0014] Example 1 Medium composition (g / l):

[0015] glucose 3.5 NaNO 3 0.30 CaCl 2 ·2H 2 O 0.50

[0016] MgSO 4 ·7H 2 O 0.075 K 2 HPO 4 0.075 NaCl 0.025

[0017] KH 2 O 4 0.075 ZnSO 4 ·7H 2 O 0.0082 MnCl 2 ·4H 2 O 0.0014

[0018] MoO 3 0.00071 CuSO 4 ·5H 2 O 0.00157 Co(NO 3 ) 2 ·6H 2 O0.0005

[0019] H 3 BO 3 0.0114 EDTANa 2 0.05 FeSO 4 0.005

[0020] KOH 0.031

[0021] pH: adjust to 6.5 with 0.1M hydrochloric acid

[0022] Inoculate Haematococcus pluvialis (cell density 2.5×10) in a 250 ml Erlenmeyer flask containing 30 ml of the above medium 5 cells / ml) and Phaffia rhodozyma (cell density 3.1×10 5 cells / ml) 3ml each, and compare with the two separately cultured. The inoculum volume of the two cultured separately is also 3ml. Place the Erlenmeyer flask in a constant temperature shaker with light. The temperature was 23.8°C, the rotation speed was 100 rpm, the light was 35001x, and the culture was 96 hours.

[0023] Extraction and de...

example 2

[0025] Example 2 The nitrogen source NaNO in the medium 3 Doubled the concentration (0.6g / l), other conditions remain unchanged, the astaxanthin yields of Haematococcus pluvialis and Phaffia rhodozyma mixed culture, Haematococcus pluvialis and Phaffia rhodozyma alone culture are respectively : 7.80mg / l, 2.05mg / l, 1.69mg / l.

example 3

[0026] Example 3 Adjust the glucose concentration in the medium to 12g / l, and other conditions remain unchanged, carry out mixed culture of Haematococcus pluvialis and Phaffia rhodozyma, astaxanthin cultured by Haematococcus pluvialis and Phaffia rhodozyma alone The yields were 6.06mg / l, 2.1lmg / l, 1.98mg / l.

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Abstract

A process for preparing astaxanthin includes such steps as preparing special culture medium, loading it in reactor, inoculating algae including Haematococcacea able to generate astaxanthin and yeast in it, culturing and fermenting. Its advantages are low cost, and high conversion rate of saccharide, biomass and output of astaxanthin.

Description

Technical field [0001] The invention relates to a method for producing astaxanthin by mixed culture and fermentation of astaxanthin-producing algae and yeast, and belongs to a biological method for producing astaxanthin. Background technique [0002] Astaxanthin is a kind of secondary carotenoid with strong antioxidant properties, and has a wide range of application prospects in medicine, food, and health products. [0003] Algae such as Haematococcus pluvialis, Chlorococcum sp, and yeast such as Phaffia rhodozyma, Phodotorula rubra, and Rhodotorula glutinis can synthesize shrimp They are the main microbial source for the production of astaxanthin using biotechnology. Haematococcus pluvialis and Chlorococcus pluvialis can carry out photosynthetic autotrophic and chemical energy heterotrophic metabolism at the same time in the light, suitable for slightly alkaline environment, slow growth rate, high astaxanthin content; Phaffia rhodozyma, deep red Y...

Claims

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Application Information

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IPC IPC(8): C12P23/00
Inventor 赵学明董庆霖
Owner TIANJIN UNIV
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