Culture solution of nitrosobacteria as well as preparation method and culture method thereof

A technology for nitrifying bacteria and culture solution, applied in biochemical equipment and methods, microorganism-based methods, bacteria, etc., can solve the problems of bacterial strains susceptible to contamination by miscellaneous bacteria, biased nutrient concentration ratio, and lack of bacteria, etc. The effect of improving reproduction and survivability, increasing reproduction and survivability, and reducing the chance of sedimentation failure

Inactive Publication Date: 2014-07-02
SOUTHEAST UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, due to the high sensitivity of this strain to environmental changes, the cultivation of pure strains is difficult and demanding, and problems such as slow bacterial growth, low density, low activity, and easy contamination by bacteria often occur during the cultivation process. , and even the situation that it cannot be cultiv

Method used

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Examples

Experimental program
Comparison scheme
Effect test

example 1

[0051] Preparation of culture medium: Prepare two glass containers of appropriate volume, two 250mL culture bottles (high temperature and high pressure resistant, sterilized), 100-1000μL pipette gun (including sterilized tip) and 1000-5000μL pipette One for each gun, 1000mL measuring cylinder, etc.; use the measuring cylinder to measure 1000mL of ultrapure water, and in the above glass container, weigh the following medicines in order to dissolve (dissolve the next medicine successively after the previous medicine is completely dissolved): 1.00g (NH 4 ) 2 SO 4 , 0.15gMgSO 4 ·7H 2 O, 0.01gCaCl 2 2H 2 O, 0.07gK 2 HPO 4 , 2.00gC 9 h 20 N 2 o 4 S (EPPS), and stirred by a magnetic stirrer at the same time, then add 800 μL SolutionA, 800 μL SolutionB, 400 μL SolutionC and 400 μL SolutionD respectively, after all the drugs are completely dissolved, wash with 40% KHCO 3 Adjust the pH to 6.90, wrap the mouth of the bottle with tinfoil, sterilize at 120°C for 30 minutes under...

example 2

[0055] Preparation of culture medium: Prepare two glass containers of appropriate volume, two 250mL culture bottles (high temperature and high pressure resistant, sterilized), 100-1000μL pipette gun (including sterilized tip) and 1000-5000μL pipette One for each gun, 1000mL measuring cylinder, etc.; use the measuring cylinder to measure 1000mL of ultrapure water, and in the above glass container, weigh the following medicines in turn to dissolve (dissolve the next medicine one by one after the previous medicine is completely dissolved): 1.20g (NH 4 ) 2 SO 4 , 0.17gMgSO 4 ·7H 2 O, 0.015gCaCl 2 2H 2 O, 0.08gK 2 HPO 4 , 2.25gC 9 h 20 N 2 o 4 S (EPPS), and stir with a magnetic stirrer at the same time, then add 900 μL SolutionA, 900 μL SolutionB, 450 μL SolutionC and 450 μL SolutionD respectively, after all the drugs are completely dissolved, wash with 40% KHCO 3 Adjust the pH to 6.95, wrap the mouth of the bottle with tinfoil, sterilize at 120°C for 30 minutes, and coo...

example 3

[0059] Preparation of culture medium: Prepare two glass containers of appropriate volume, two 250mL culture bottles (high temperature and high pressure resistant, sterilized), 100-1000μL pipette gun (including sterilized tip) and 1000-5000μL pipette One for each gun, 1000mL measuring cylinder, etc.; use the measuring cylinder to measure 1000mL of ultrapure water, and in the above glass container, weigh the following medicines in turn to dissolve (dissolve the next medicine one by one after the previous medicine is completely dissolved): 1.32g (NH 4 ) 2 SO 4 , 0.2gMgSO 4 ·7H 2 O, 0.02g CaCl 2 2H 2 O, 0.09gK 2 HPO 4 , 2.5gC 9 h 20 N 2 o 4 S (EPPS), and stir with a magnetic stirrer at the same time, then add 1000 μL SolutionA, 1000 μL SolutionB, 500 μL SolutionC and 500 μL SolutionD respectively, after all the drugs are completely dissolved, wash with 40% KHCO 3 Adjust the pH to 6.93, wrap the mouth of the bottle with tinfoil, sterilize at 120°C for 30 minutes, and coo...

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Abstract

The invention relates to a culture solution of a typical nitrosomonaseuropaea and a culture method thereof. The culture method comprises the steps of preparing the culture solution, activating the bacteria and culturing. By adopting the culture solution and the method, the outstanding problems that the existing bacteria is difficult to culture, the requirement is high, the bacteria is easy to be polluted in the culture process and slow to grow, and the like can be solved. The culture solution comprises nutritional substances needed by the growth of the bacteria, a biological buffering agent and a pH indicating agent, and the culture solution can promote the growth of the bacteria, improve the propagation capacity and survival capacity of the bacteria and can judge and adjust the growth state and activity of the bacteria according to the color variation of the indicating agent; in the culture process, the bacteria is activated, so that the activity and the growth rate of the bacteria can be further increased, the culture period can be greatly shortened, the pH can be monitored and adjusted in real time, the survival capacity of the bacteria can be further improved, and the culture efficiency can be improved.

Description

technical field [0001] The invention belongs to the field of environmental engineering microorganisms, in particular to a culture solution and a culture method of a typical ammonia oxidizing bacterium (N. europaea). Background technique [0002] At present, the nitrogen and phosphorus removal process of domestic sewage is mainly removed through the absorption and transformation of microorganisms. The ammonia nitrogen in the sewage is first oxidized into NO sequentially under the action of ammonia oxidizing bacteria and nitrosifying bacteria. 2- and NO 3- , then NO 3- Converted to N under the action of denitrifying bacteria 2 be removed. [0003] N.europaea is a typical and widespread chemoautotrophic ammonia-oxidizing bacteria in sewage biological denitrification treatment systems, and is very sensitive to environmental changes and toxic stress of pollutants. The general pathway of oxidative metabolism in the nitrogen cycle mainly includes the sequential oxidation of amm...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12R1/01
Inventor 余冉吴俊康陈良辉刘美婷
Owner SOUTHEAST UNIV
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