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Carbon compensator for large-scale culturing micro-algae, its utilization and use

A large-scale cultivation and carbon replenishment technology, which is applied in enzymology/microbiology devices, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the waste of carbon sources, the inability to be used, the waste of water resources and carbon sources, etc. problems, to achieve the effect of prolonging the gas-liquid contact time, reducing production costs and improving absorption rate

Inactive Publication Date: 2007-06-20
INST OF PROCESS ENG CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

NaHCO 3 As a carbon source in the culture medium can not be fully utilized, with the HCO 3 - dissociation and utilization, the pH value of the culture medium gradually increases, which is not suitable for algae to continue to grow
More than half of NaHCO 3 converted to Na 2 CO 3 Can not be used, resulting in a large waste of carbon sources
Therefore, with NaHCO 3 Cultivating microalgae for carbon sources will result in a large waste of water resources and carbon sources, and it is also an important reason for the high cost of microalgae production. For example, to produce 1 ton of spirulina (calculated by dry weight), it takes 8 tons of NaHCO 3 and 1000 tons of water and about 3 tons of other nutrients

Method used

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  • Carbon compensator for large-scale culturing micro-algae, its utilization and use
  • Carbon compensator for large-scale culturing micro-algae, its utilization and use
  • Carbon compensator for large-scale culturing micro-algae, its utilization and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] Spirulina culture is carried out in the raceway culture tank. The runway pool has a circumference of 200 meters, a width of 2 meters and a water depth of 15 centimeters. The driving device of the culture medium is a steel impeller driven by a motor. The algal species were from the Institute of Process Engineering, Chinese Academy of Sciences, the species was Spirulina Platensis, the medium was Zarrouk medium, and the initial concentration of sodium bicarbonate in the culture medium was 0.1mol / L.

[0048] Automatic carbon replenishment is adopted, the automatic control method is shown in CN200410009360.4, and the implementation scheme system is shown in Figure 2. Wherein, the pH sensor 10 is a commercially available steam sterilizable pH electrode, the control device 11 is a pH meter with switch control, CO 2 The gas source 12 is pure carbon dioxide gas, the actuator 4 is a two-position normally closed solenoid valve (diameter 8 mm), and the gas distributor 7 is a glas...

Embodiment 2

[0050] With embodiment 1, but the initial concentration of sodium bicarbonate is changed into 0.2mol / L, and the flow rate (executive agency 4 is opened) of carbon dioxide gas in each trap type container is 4.0L / min (standard condition). Continuously cultivated for 2 months, the yield of algae per unit area reaches 9.5g / m 2 .d. The composition and content of conventional components, amino acids, fatty acids and carotenoids obtained from Spirulina algae powder are basically consistent with those reported in the literature. Each kilogram of algal powder (dry weight) consumes about 2.62 kilograms of carbon dioxide.

Embodiment 3

[0052] Same as Example 1, the difference is that the pH control range is 8.5-11.0, and the flow rate of carbon dioxide gas in each well-type container (4 open actuators) is 4.0 L / min (standard condition). Continuously cultivated for 2 months, the yield of algae per unit area reaches 9.0g / m 2 .d. The composition and content of conventional components, amino acids, fatty acids and carotenoids obtained from Spirulina algae powder are basically consistent with those reported in the literature. Each kilogram of algal powder (dry weight) consumes about 2.5 kilograms of carbon dioxide.

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Abstract

A carbon supplementing apparatus for supplying carbon dioxide efficiently in micro-algae culturing tank, its usage and use are disclosed. A vertical baffle plate is installed above in the container with 10-50 cm gap, gas distributor is arrange at bottom of tank, the apparatus is built-in the bottom of tank, whose upper edge is aligned with the tank bottom, baffler plate is above culturing level of tank so as to pass culturing fluid to the apparatus with agitator driven. It has longer vapor-liquid contact time, more vapor-liquid contact area and higher carbon dioxide efficiency.

Description

technical field [0001] The invention belongs to the field of large-scale cultivation of microalgae, and in particular relates to a carbon replenishing device for efficiently supplying carbon dioxide during large-scale cultivation of microalgae in an open pool, and a method and application thereof. technical background [0002] Microalgae has high application value. Microalgae cells are rich in various high value-added biological substances such as protein, amino acid, carbohydrate, vitamin, antibiotic, high unsaturated fatty acid, polysaccharide and pigment, and some microalgae also have very High hydrocarbon production capacity (such as botrytis), has a good application prospect in energy utilization. In today's increasingly severe global food and energy crisis, the development and utilization of microalgae biological resources undoubtedly have important social value and economic significance. [0003] The large-scale culture of microalgae can be divided into closed and op...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/06
CPCC12M21/02C12M27/20C12M23/18C12M27/06C12M29/08
Inventor 丛威苏贞峰康瑞娟杨成砚蔡昭铃
Owner INST OF PROCESS ENG CHINESE ACAD OF SCI
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