Establishment method and application of mass spectrum database of prohibited colorant in foods

A technology for establishing methods and colorants, which is applied in special data processing applications, electrical digital data processing, measuring devices, etc., can solve problems such as inability to handle, inability to detect, and identification of detection institutions, and achieve the effect of improving product quality

Inactive Publication Date: 2013-08-07
JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
View PDF2 Cites 17 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Even for suspicious products, it is difficult for testing agencies to identify them technically, or even impossible to detect them at all.
This leads to the phenomenon that cannot be dealt with due to lack of basis in food supervision.

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Establishment method and application of mass spectrum database of prohibited colorant in foods
  • Establishment method and application of mass spectrum database of prohibited colorant in foods
  • Establishment method and application of mass spectrum database of prohibited colorant in foods

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] In this example, 102 colorants shown in Table 1 were selected for sorting and analysis, and a Q-TOF mass spectrum database was established according to the method of the present invention. Among them, there are 16 edible pigments and 86 industrial dyes banned in food, including acid dyes, basic dyes, direct dyes, disperse dyes, reactive dyes, and mordant dyes.

[0038] Enter the collected information such as the compound name, CAS number, neutral molecular formula, structural formula and other information of the colorant shown in Table 1 into the Edit Compound Information entry in the Personal Compound Database and Libarary Mangager (PCDL) software; then , the collected library-building compound standards were prepared into standard stock solutions and working solutions, respectively, and the chromatographic conditions and mass spectrometry conditions were adjusted. The method is as follows:

[0039] [0035] Instruments and equipment: 1200RRLC high performance liquid...

Embodiment 2

[0061] After the establishment of the colorant mass spectrum database described in Example 1, it was found that the exact molecular weights of some colorants with similar properties were very close. In order to distinguish such compounds, this example carried out chromatographic separation by adopting chromatographic column, adjusting the proportion and type of mobile phase. First, the colorants were grouped according to their molecular weight, and were summarized into eight groups of compounds, as shown in Table 3.

[0062] The mass spectrometry conditions for the analysis of the eight groups of compounds are as follows:

[0063] Jet stream ESI source; nozzle voltage is 1000V, capillary voltage is 3500V, collision voltage is 130V, cone voltage is 65V; drying gas temperature is 350°C; drying gas flow rate is 5L / min; atomizer pressure is 45psi; sheath gas The temperature is 350°C; the sheath gas flow rate is 11 L / min; the mass spectrum acquisition mode is MS mode, the mass spe...

Embodiment 3

[0085] Example 3 Qualitative and quantitative detection of Congo red in beef

[0086] The identification method of illegally added Congo red in food discussed in this example is expected to provide a good solution for the identification and quantification of illegal additives, and to provide guarantee for food quality control and safety evaluation.

[0087] Instruments and Materials:

[0088] 1200RRLC high performance liquid chromatograph connected in series with 6530 quadrupole-time-of-flight mass spectrometry, MassHunter chromatography workstation and PCDL software have built-in colorant mass spectrum database (Agilent, USA); XW-80A vortex mixer (Shanghai Qingpu Huxi) Instrument Factory); Avanti J-E Centrifuge (Beckman Coulter, USA); 5418R Centrifuge (Aibend China Co., Ltd.); XS-205DU Electronic Analytical Balance (Mettler-Toledo, USA); FW100 high-speed universal crusher (Tianjin Test Instrument Co., Ltd.).

[0089] The beef samples were commercial samples.

[0090] ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

PUM

No PUM Login to view more

Abstract

The invention aims to provide an establishment method and application of a mass spectrum database of a prohibited colorant in foods. The method provided by the invention comprises the steps of: (1) establishing a first-level mass spectrum database and a second-level mass spectrum database of the colorants; and (2) separating the colorants with similar properties in the established mass spectrum databases. According to the establishment method and the application, the technical evaluation foundations are provided for the law enforcement; a purpose of fast screening the unknown prohibited added colorants is achieved by virtue of the mass spectrum databases; and the establishment method and the application can be helpful for strengthening the food detection of markets and carrying out preventive measures to effectively protect life safety of people.

Description

technical field [0001] The invention belongs to the field of food safety, and in particular relates to a method for establishing a mass spectrum database of prohibited colorants in food and its application. Background technique [0002] The coloring agent in food originally refers to food coloring, which is a coloring substance that directly colors food to improve the sensory color of food. There are two types of food coloring that are commonly used to allow addition: food natural coloring and food synthetic coloring. Edible natural colors come from natural sources, mostly edible sources. It is mainly extracted from plant tissues, but some are also some pigments from animals and microorganisms. Natural pigments are highly safe and have soft and natural colors. Many of them also have high nutritional value and pharmacological effects, which are beneficial to human health. With the improvement of people's health care awareness, people's advocating nature is increasing day b...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to view more

Application Information

Patent Timeline
no application Login to view more
Patent Type & Authority Applications(China)
IPC IPC(8): G06F17/30G01N30/02
Inventor 徐春祥林慧颜春荣张征朱晓军
Owner JIANGSU SUPERVISION & INSPECTION INST FOR PROD QUALITY
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Try Eureka
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap