GC-MS non-target method used for rapid screening of plant differential metabolites

A GC-MS and differential technology, applied in the fields of flavors and fragrances, metabolomics research, can solve the problems of missing and clarifying the aroma and taste components, etc., so as to reduce the workload, quickly screen and improve the analysis efficiency effect

Active Publication Date: 2017-07-21
NINGXIA MEDICAL UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are a large number of false positives and false negatives in these methods. As a result, when using these methods for analysis, it is inevitable to miss important substance information, which leads to the fact that the differential metabolites screened cannot effectively represent group differences. Aroma and flavor ingredients don't help much

Method used

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  • GC-MS non-target method used for rapid screening of plant differential metabolites
  • GC-MS non-target method used for rapid screening of plant differential metabolites
  • GC-MS non-target method used for rapid screening of plant differential metabolites

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Embodiment 1

[0027] A total of 30 tea samples from two regions were frozen at -80°C to remove water, then pulverized by a pulverizer, passed through a 60-mesh sieve, and stored at -20°C in the dark. Transfer the samples to room temperature for half an hour before analysis.

[0028] For each tea sample, 100 μl of a deuterated acetophenone solution (constant volume in methanol) with a concentration of 100 μg / ml was added as an internal standard, and 5 ml of dichloromethane was added as an extraction solution to extract the chemical components of the plant sample. After vortexing for 3 min and ultrasonication for 1 h, centrifuge at 13000 r / min for 10 min, take 1 ml of supernatant, add 100 μl of BSTFA derivatization reagent, and derivatize at 60°C for 1 h. Then enter GC-MS for analysis.

[0029] Set the GC-MS analysis conditions:

[0030]Chromatographic conditions: the chromatographic column is an Agilent DB-5MS column (60m×2.5mm, i.d., 2.5μm). The temperature was programmed to rise, the in...

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Abstract

The invention discloses a GC-MS non-target method used for rapid screening of plant differential metabolites, and belongs to the technical field of flavours and fragrances, and metabonomics study technology. According to the GC-MS non-target method, modern GC-MS full scanning technology is adopted to obtain chemical ingredient information of volatile substances in plant; multidimensional Gaussian smoothing is adopted to extract substance information in a TIC picture; dynamic programming algorithm is adopted to calibrate chromatographic peak time shifting among samples, realize aligning of chromatographic peaks belongs to one same substance, achieve comparability of substance information among different samples; nearest neighbor-clustering algorithm is adopted to realize compound registration; and at last variance analysis is adopted to screen compounds with significant difference from different groups. According to the GC-MS non-target method, compound mass spectrum information is output so as to obtain a MSP file; and the MSP file can be input into the NIST database to search compounds. The GC-MS non-target method can be used for locking compounds with dissimilarities in plant samples rapidly, and is suitable for rapid analysis determination of a large amount of samples.

Description

technical field [0001] The present invention utilizes GC-MS technology to screen the volatile substances in different groups of plant samples, and specifically relates to the technical fields of flavors and fragrances and metabolomics research. Background technique [0002] The aroma and taste substances in natural plant flavors and fragrances are mainly secondary metabolites in the natural growth environment of plants. Due to differences in factors such as regions, climates, and varieties, even if the finished flavors and fragrances are made from the same plant using the same process, their There is also a big difference in the senses presented. By studying the differential metabolites in plants, the chemical components that play an important role in the external sensory quality can be screened out, which has important practical significance for the processing of flavors and fragrances. Since there are thousands of chemical components in plants, using GC-MS non-target meta...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/02G01N30/06
Inventor 于永杰佘远斌付海燕
Owner NINGXIA MEDICAL UNIV
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