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Major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as detection primer and application thereof

A technology of molecular markers and detection primers, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, recombinant DNA technology, etc., can solve the problems of long cycle, occupation of cultivation space, repetitive and cumbersome processes, etc., and achieve low cost and high results. Accurate and easy experimental operation

Active Publication Date: 2020-11-24
WUHAN ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The cycle is long, the process is repetitive and cumbersome, it takes up a lot of cultivation space, the efficiency is low, and the labor cost is high

Method used

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  • Major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as detection primer and application thereof
  • Major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as detection primer and application thereof
  • Major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as detection primer and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0041] Example 1 Using the SLAF-seq method to determine the major QTL candidate regions and the development of linkage markers controlling the safflower and white flower traits of lotus

[0042] 1. Construct segregated populations of lotus flower color traits

[0043] In this example, the F2 genetic population was constructed with the lotus "Jumba" as the male parent and the lotus "Gypsophila" as the female parent. Among them, the male parent is white flowers, and the female parent is red flowers.

[0044] 2. Field identification of lotus flower color phenotype

[0045] The petal color of the parents, F1 and each F2 individual plant was identified and counted in the field.

[0046] 3. Genomic DNA extraction and SLAF library construction

[0047] One lotus seed was sown in each sample of 2 parents and 175 offspring in the F2 genetic segregation population, and young leaves were taken after germination, and genomic DNA was extracted by CTAB method. The DNA quality and concen...

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Abstract

The invention discloses a major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as a detection primer and application thereof. The invention firstly provides a majorQTL site for controlling the flower color traits of Nelumbo nucifera, and the major QTL site is positioned in a sixth linkage group and between two SNP markers; the major QTL is closely linked with the SNP molecular marker, has a relatively high contribution rate to the flower color traits of the Nelumbo nucifera, participates in regulating and controlling the phenotype of the red or white flowerof the Nelumbo nucifera, and can be used for map-based cloning mining of functional genes for controlling the flower color traits and molecular marker-assisted selection. The invention further provides an SNP molecular marker closely linked with the major QTL, and the SNP molecular marker is used for efficiently selecting Nelumbo nucifera varieties with target flower colors and molecular marker assisted breeding of Nelumbo nucifera with seeds and Nelumbo nucifera with roots. The invention further provides a PARMS primer group for detecting the SNP marker and a method for identifying the red and white flower traits of the Nelumbo nucifera, so that the flower color genotype data of the Nelumbo nucifera can be analyzed and obtained only through PCR amplification and without electrophoresis detection.

Description

technical field [0001] The invention relates to SNP molecular markers and detection primers, in particular to main effect QTL for controlling the traits of lotus safflower and white flowers, SNP molecular markers, detection primers and their application in molecular assisted breeding, belonging to the field of lotus molecular assisted breeding. Background technique [0002] Lotus (Nelumbo nucifera), as a garden aquatic plant of the genus Nelumbo, can be divided into three types: seed lotus, lotus root and flower lotus after long-term cultivation and domestication. Zilian mainly produces lotus seeds, with luxuriant flowering and many red flowers, but the rhizomes are slender; lotus mainly produces underground rhizomes, with few flowers and all of them are white. Relatively speaking, Hualien has the most abundant flower shapes and colors and has high ornamental value, but some varieties have a low seed setting rate. Lotus is one of the top ten traditional famous flowers in Ch...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6895C12Q1/6858C12Q2600/124C12Q2600/156C12Q2531/113C12Q2563/107Y02A50/30
Inventor 刘正位朱红莲李静柯卫东彭静匡晶季群
Owner WUHAN ACADEMY OF AGRI SCI
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