41results about How to "Experimental operation is fast" patented technology

The invention relates to the technical field of electric power, in particular to a system and method for testing the electric conductivity of a metal wire. The system comprises a direct-current power supply, a wire clamping system and a test system. The output end of the direct-current power supply is in series connection with the metal wire to be tested through a fixture. The metal wire to be tested is connected to an optical platform through a fixture. A first camera and a second camera are respectively and electrically connected with a computer. A multiple-pass digital multimeter V is connected with the metal wire to be tested and is further electrically connected with the computer. A multiple-pass digital multimeter A is connected with the metal wire to be tested through the direct-current power supply. Due to the adoption of the system and method for testing the electric conductivity of the metal wire, the electric conductivity of a metal wire of any length and section shape can be tested without considering the effect of the temperature on the resistance of the wire to be tested, and experiments are easy and quick to operate. The system and method greatly improve working efficiency, obviously improve testing accuracy and are suitable for all kinds of novel energy-saving metal wires.

The invention provides a method capable of simultaneously detecting varieties of pesticide residues in an aquatic product. The method capable of simultaneously detecting varieties of pesticide residues in the aquatic product comprises the following steps of extracting varieties of pesticide residues in the to-be-detected aquatic product, purifying a pesticide residue-containing extract, performing qualitative and quantitative analysis on the purifying liquid of the pesticide residue-containing extract through LC-MS/MS (liquid chromatography-mass spectrography)/(mass spectrography) and the like. According to the method capable of simultaneously detecting varieties of pesticide residues in the aquatic product, the function of separating and detecting all the pesticide residues by one step is realized, so that the efficiency of a sample pretreatment and analysis process is improved, the flexibility is high, and the detection cost is reduced to one tenth of the detection cost of the conventional method.

The invention discloses a method for simultaneously measuring residual quantity of three types of herbicide in vega soil, belonging to the technical field of physicochemical detection of pesticide residue in vega soil. Specifically, acetonitrile is used for extraction, Florisil is carried out with solid phase extraction, a gas chromatograph is matched with an electron capture detector to directly measure the residual quantity of three types of herbicide, such as propanil, Rimsulfuron, quizalofop-p-ethyl and the like in vega soil. The invention overcomes the restriction of the defect and the condition of the prior art to optimize the pretreatment method and the instrument detection condition of vega soil samples. Compared with the prior art, the invention has the following favorable advantages that the invention can detect three types of herbicide all at once and has the advantages of simple pretreatment process, high detection sensitivity and good accuracy and reproducibility.

The invention discloses a honeysuckle quality evaluation method. According to the honeysuckle quality evaluation method, chlorogenic acid, galuteolin and six iridoid glycoside ingredients in honeysuckle are determined, wherein the six iridoid glycoside ingredients are loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal. The honeysuckle quality evaluation method disclosed by the invention has the advantages that the iridoid glycoside ingredients in the honeysuckle are included in a honeysuckle quality evaluation system for the first time, six representative iridoid glycoside ingredients in the honeysuckle are selected and determined at the same time for guaranteeing reliability and effectiveness of the quality evaluation method, and a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) is established for the first time and used for detecting the six iridoid glycoside ingredients of different structures, so that reliability and effectiveness of honeysuckle quality evaluation are obviously improved, quality control level of traditional Chinese medicine honeysuckle and related patent medicines is further improved, and the honeysuckle quality evaluation method disclosed by the invention is significant on guidance of clinical application.

The invention discloses a major QTL and SNP molecular marker for controlling Nelumbo nucifera color traits as well as a detection primer and application thereof. The invention firstly provides a majorQTL site for controlling the flower color traits of Nelumbo nucifera, and the major QTL site is positioned in a sixth linkage group and between two SNP markers; the major QTL is closely linked with the SNP molecular marker, has a relatively high contribution rate to the flower color traits of the Nelumbo nucifera, participates in regulating and controlling the phenotype of the red or white flowerof the Nelumbo nucifera, and can be used for map-based cloning mining of functional genes for controlling the flower color traits and molecular marker-assisted selection. The invention further provides an SNP molecular marker closely linked with the major QTL, and the SNP molecular marker is used for efficiently selecting Nelumbo nucifera varieties with target flower colors and molecular marker assisted breeding of Nelumbo nucifera with seeds and Nelumbo nucifera with roots. The invention further provides a PARMS primer group for detecting the SNP marker and a method for identifying the red and white flower traits of the Nelumbo nucifera, so that the flower color genotype data of the Nelumbo nucifera can be analyzed and obtained only through PCR amplification and without electrophoresis detection.

The invention discloses a method for synchronously extracting and purifying various chlorinated polycyclic aromatic hydrocarbons in soil, which belongs to the field of detection of the chlorinated polycyclic aromatic hydrocarbons. The method comprises the following steps: firstly removing moisture in a soil sample by virtue of a freezing dryer, grinding the soil sample, and filtering the ground soil sample by virtue of a 200-mesh sieve for later use; and extracting a target matter in the soil sample by utilizing an acceleration solvent extractor, purifying the target matter in a gel permeation chromatography, finally concentrating the target matter and sizing the target matter. According to the method, a preprocessing method of the chlorinated polycyclic aromatic hydrocarbons in the soil is established, less solvent is used in the entire process, the automation degree is high, less time is used, the safety is high, fewer steps are used, and various chlorinated polycyclic aromatic hydrocarbons in the soil can be accurately, qualitatively and quantitatively determined. The chlorinated polycyclic aromatic hydrocarbons in the soil are quantitatively detected by utilizing the gas phase chromatography-mass spectrometry, the detection limit is low (when S/N is equal to 3, the detection limit is 2.6 to 25pg/g), the precision is high (RSD=6.30 to 12.0 percent), the blank mark recovery rate is 64.1 to 109.2 percent, the synchronous analysis and detection of various trace chlorinated polycyclic aromatic hydrocarbons in the complicated environment medium such as the soil can be realized, sensitivity and accuracy can be realized, and the weaknesses of the prior art can be overcome.

The invention discloses a method for simultaneously measuring the residue contents of five weedicides with different structures in soil, and belongs to the technical field of physicochemical detection of pesticide residues in the soil. Specifically, the residue contents of three types of five weedicides with different structures in the soil are directly measured by a high-performance liquid chromatographic (HPLC) instrument by combining dispersive solid-phase extraction (DSPE) with dispersive liquid-liquid microextraction (DLLME). According to the method, the defects and the condition limitations in the prior art are overcome, a method for pre-treating a soil sample and the detection conditions of the instrument are optimized, a DSPE-DLLME-HPLC method is built for simultaneously detecting three types of five weedicides (metsulfuron methyl, bensulfuron methyl, prometryn, atrazine and quinclorac) with different structures in the soil, the linear correlation coefficients are all more than 0.99, the detection limit is 1.5-3.1 ngg<-1>, the average recovery rate is 74.9-90.1%, the relative standard deviation (RSD) is 4.0-5.7%, both the accuracy and the precision meet the residue detection requirements, and the method has the characteristics of low organic solvent consumption, good purification and concentration effects, high sensitivity, simplicity for performing and the like.

The invention relates to a detection and identification kit of a composite PCR of wheat stripe rust fungus and leaf rust fungus, which belongs to the technical fields of biotechnology, crop disease control and plant quarantine. The kit detects the specific genome DNA sequences of the wheat stripe rust fungus 171 bp and the leaf rust fungus 226 bp simultaneously in a primary PCR reaction by three primers from a fungal beta-tubulin gene conserved sequence and wheat stripe rust fungus and leaf rust fungus specific sequences. The invention provides supplement and improvement on the wheat stripe rust fungus and leaf rust fungus specific primer detecting method, and is suitable for the diagnostic detection of the stripe rust fungus and the leaf rust fungus in an infected wheat leaf before disease symptoms appear. Composite PCR technology is adopted in the kit, and in a PCR-SMART reaction solution, the three primers are amplified simultaneously, so the detection accuracy is increased, the detection time is saved, the operation is simple, and the kit can be applied and popularized at plant protection and plant quarantine departments in China.

The disclosed preparation method for an electrode chemical modified by O-carboxylmethychitose covalence bond comprises: dipping glass-carbon electrode into O-carboxylmethychitose-formic acid solution for modification, taking out to clean with formic acid and distilled water. This invention can improve detection sensitivity for DA, can eliminate effect of AA and UA, and has long lifetime.

The invention provides a method for determining the residual amount of three types of phenoxyalkanoic acid pesticides in tobaccos through an extraction-purification synchronization method. The methodis characterized by comprising the following steps: extracting a sample by utilizing a 0.5mol/L hydrochloric acid water solution; carrying out back extraction by utilizing dichloromethane; determiningthe residual amount of the pesticides including 2,4-D, 2,4,5-T and dicamba by utilizing an ultra performance liquid chromatography tandem mass spectrometry. According to the method provided by the invention, a standard working solution is prepared by utilizing a blank matrix and the influences on a matrix effect are overcome and the disadvantages of a sample treatment method in the prior art aremade up; a sample pre-treatment method and an instrument detection condition are optimized aiming at the tobacco sample; compared with the prior art, the method provided by the invention has the following good effects that a sample pre-treatment process of the method is simple and rapid and does not need derivatization; the method is green and environmentally friendly and has the advantages of accuracy in operation, high sensitivity and good repeatability.

The invention provides a method and device for measuring the absolute concentration of singlet-state oxygen (O2(al(delta)). The device comprises a static pool, an infrared detection system and a data acquisition system, wherein the infrared detection system is composed of a chopper, a lens, a narrow-band interference optical filter, an infrared detector and a phase-locked amplifier. In the invention, by monitoring the infrared radiation attenuation change of O2(al(delta)) at 1.27 microns in the static pool and combining the dynamic model analysis in the static pool, the absolute response coefficient of the infrared detection system can be automatically calibrated, and the absolute concentration of O2(al(delta)) can be further measured in real time. Moreover, the infrared attenuation method of the static pool can be applied to the real-time measurement for any flowing system containing O2(al(delta)) by use of a 'flowing-stopping' technology.

The invention relates to a typing method for distinguishing different strains of cronobacter through performing single enzyme digestion on gluA gene based on RsaI, and belongs to the technical field of microbiological examination of food. Operation steps of the method disclosed by the invention are divided into two stages: 1, in the first stage, specific primer amplification on the cronobacter isperformed, so that gluA gene fragments of six different strains of the cronobacter are obtained; and 2, in the second stage, the specific gluA gene fragments of the cronobacter, which is amplified inthe first stage, are subjected to RsaI enzyme digestion, so that differential PCR-RFLP fingerprint graphs of the six different strains of the cronobacter are obtained, and the different strains of thecronobacter are further distinguished. A large quantity of examples of strains of the cronobacter verify that by adopting the molecular typing method, the differential enzyme digestion polymorphic graphs of the different strains can be effectively obtained, and the six different strains of the cronobacter can be quickly and accurately distinguished. The molecular typing method disclosed by the invention is used for distinguishing the strains of the cronobacter, and has an important practical meaning on accurately tracing pollution of the cronobacter in the food.

The invention discloses a method for detecting genotoxic impurities in an indobufen bulk drug. According to the method, octadecyl silane bonded silica gel or octyl bonded silica gel is used as a stationary phase for chromatographic separation of genotoxic impurities, a mass spectrum detector is used for analysis and detection, and the genotoxic impurities 2-(4-amino) phenylbutyric acid and/or 2-(4-nitro) phenylbutyric acid can be detected independently or simultaneously. According to the method, the genotoxic impurity 2-(4-amino) phenylbutyric acid and/or 2-(4-nitro) phenylbutyric acid can be independently or simultaneously and effectively separated, the content of the genotoxic impurity can be accurately determined, the specificity is high, the sensitivity is high, and the method has extremely important significance on quality control and safety guarantee of indobufen bulk drugs.