Method for simultaneously measuring six iridoid glycoside ingredients in honeysuckle flowers

Abstract

The invention discloses a method for simultaneously measuring six iridoid glycoside ingredients in honeysuckle flowers. The six iridoid glycoside ingredients, namely, loganic acid, morroniside, loganin, chiratin, secoxyloganin and secologanin dimethyl acetal, of different structures in honeysuckle flowers are measured simultaneously based on a DSPE-HPLC method for the first time; by means of the method, the linearly dependent coefficients are larger than 0.999, the detection limit is 0.026-0.057 micrograms / mL, the recovery rates of all target ingredients range from 90.5% to 102.1%, the relative standard deviation (RSD) ranges from 0.85% to 1.74%, and the method has the advantages that the using amount of an organic solvent is small, the purification and concentration effects are good, the recovery rates of targets are high, the sensitivity is high, and operation is easy. The fast and efficient extraction, purification and analysis method suitable for iridoid glycoside ingredients in medical materials such as honeysuckle flowers is developed, and has great significance on further developing the acting mechanism of iridoid glycoside ingredients, establishing quality standards, increasing medical material and related patent medicine quality control levels and guiding clinical application.

Description

technical field [0001] The invention belongs to the technical field of medical analysis, and in particular relates to a method for simultaneously determining six iridoid glycoside components in honeysuckle. Background technique [0002] Honeysuckle, also known as double flower, two flower, soil silver flower, honeysuckle flower, etc., is the dry flower bud or the first flower of Lonicera japonica Thunb., which is widely distributed in various provinces of my country. It has a long history of application and is commonly used in my country. One of the bulk of traditional Chinese medicinal materials, first recorded in Liang Dynasty Tao Hongjing's "Famous Doctors", sweet in taste and cold in nature, it belongs to the lung, heart and stomach meridian, has the functions of clearing away heat and detoxification, and dispersing wind-heat, mainly used for treating carbuncles and furuncles , sore throat, erysipelas, heat-toxin blood dysentery, wind-heat cold, febrile disease fever embol...

AI Technical Summary

Problems solved by technology

[0004] Because there are many types of chemical components in honeysuckle and large differences in polarity, including flavonoids, organic acids, iridoid glycosides, saponins and other chemical components; at the same time, there are also interfering components such as pigments and sugars in the extract of honeysuckle. Complex matrix

However, iridoid glycosides in honeysuckle belong to a class of components with relatively low amount and strong polarity. Using a single reflux or ultrasonic extraction technology can easily lead to complex composition of the extract and great difficulty in separation.

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