A kind of honeysuckle quality evaluation method

A honeysuckle and detection method technology, which is applied in the field of medical detection, can solve the problems of single evaluation standard components, etc., and achieve the effects of improving quality control level, high detection sensitivity, and improving reliability and effectiveness

Active Publication Date: 2018-10-26
SHANDONG ANALYSIS & TEST CENT
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there are existing research reports on the quality evaluation of honeysuckle, and most of them are carried out with reference to the Pharmacopoeia, and the evaluation standard components are relatively single.

Method used

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  • A kind of honeysuckle quality evaluation method
  • A kind of honeysuckle quality evaluation method
  • A kind of honeysuckle quality evaluation method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0031] A method for evaluating the quality of honeysuckle, comprising measuring chlorogenic acid, luteolin and six iridoid glycoside components in honeysuckle, wherein the six iridoid glycoside components are loganic acid, morroniside , loganin, dangyaoside, broken-oxygenated loganin, and bistroside dimethyl acetal; the determination methods of chlorogenic acid and luteolin were carried out in accordance with the 2015 edition of "Chinese Pharmacopoeia". The instrument materials and method steps for detecting the six iridoid glycosides are listed in detail as follows.

[0032] 1 Instruments and materials

[0033] Agilent 1260 high performance liquid chromatograph, Agilent Corporation of America; KQ2400KDE high-power numerical control ultrasonic instrument, Kunshan Ultrasonic Instrument Co., Ltd.; R201 rotary evaporator, Shanghai Shensheng Technology Co., Ltd.; BSA1245-CW precision balance, Germany Sartorius Company; Milli-Q (18.2MΩ) ultrapure water treatment system, American M...

Embodiment 2

[0050] As described in Example 1, another honeysuckle sample 2 was selected, and the experimental results are shown in Table 2.

[0051] The determination result of iridoid glycosides in the sample of table 2

[0052]

[0053] According to the method described in the 2015 "Chinese Pharmacopoeia", the chlorogenic acid and luteolin in the honeysuckle No. 2 sample were determined: the content of chlorogenic acid was 31.5 mg / g, and the content of sweet clover licorice was 0.815 mg / g.

Embodiment 3

[0055] As described in Example 1, another honeysuckle sample 3 was selected, and the experimental results are shown in Table 3.

[0056] The determination result of iridoid glycosides in the sample of table 3

[0057]

[0058] According to the method described in the 2015 "Chinese Pharmacopoeia", the chlorogenic acid and luteolin in the honeysuckle No. 2 sample were determined: the content of chlorogenic acid was 28.6 mg / g, and the content of sweet clover licorice was 0.735 mg / g.

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Abstract

The invention discloses a method for evaluating the quality of honeysuckle. The method measures chlorogenic acid, luteolin and six iridoid glycoside components in honeysuckle, wherein the six iridoid glycoside components are Loganic acid, morroniside, loganin, dangdaoside, broken-oxyloganin, and bistroside dimethyl acetal. In the present invention, for the first time, the iridoid glycosides in honeysuckle are included in the quality evaluation system of honeysuckle, and in order to ensure the reliability and effectiveness of the quality evaluation method, six representative iridoid glycosides in honeysuckle are selected for determination , and for the first time established a dispersive solid phase extraction-high performance liquid chromatography (DSPE-HPLC) method to detect these six iridoid glycosides with different structures, which significantly improved the reliability and effectiveness of the quality evaluation of honeysuckle, thereby further It has improved the quality control level of honeysuckle medicinal materials and related finished medicines, which is of great significance for guiding clinical application.

Description

technical field [0001] The invention belongs to the technical field of medical detection, and in particular relates to a method for evaluating the quality of honeysuckle. Background technique [0002] Honeysuckle, also known as double flower, two flower, soil silver flower, honeysuckle flower, etc., is the dry flower bud or the first flower of Lonicera japonica Thunb., which is widely distributed in various provinces of my country. It has a long history of application and is commonly used in my country. One of the bulk of traditional Chinese medicinal materials, first recorded in Liang Dynasty Tao Hongjing's "Famous Doctors", sweet in taste and cold in nature, it belongs to the lung, heart and stomach meridian, has the functions of clearing away heat and detoxification, and dispersing wind-heat, mainly used for treating carbuncles and furuncles , sore throat, erysipelas, heat-toxin blood dysentery, wind-heat cold, febrile disease fever embolism. [0003] There are many factor...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02
CPCG01N30/02
Inventor 王岱杰赵恒强王晓耿岩玲刘伟周冰谦
Owner SHANDONG ANALYSIS & TEST CENT
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