Method for judging strain participating in antimony reduction process in soil and key function gene of strain

Abstract

The invention discloses a method for judging a strain participating in the antimony reduction process in soil and a key function gene of the strain. After a substrate of the strain is consumed throughstarved culture, a unique metabolism substrate is added, a unique electron acceptor Sb(V) is provided, so that only one leading electron exchange process exists in a system, a microorganism metabolizes and oxidizes the organic substrate, antimony reduction is driven through coupling, and accordingly the Sb(V) obtains electrons and is reduced into an Sb(III). According to the method, the Sb(V) reduction phenomenon of an anaerobic culture system, subjected to Sb(V) stress, of the rice field soil is observed, and a DNA-SIP technology is used for identifying phylogeny information of the microorganism which can drive the Sb(V) reduction process in the culture system. According to the method, metabolism information of the antimony reduction function microorganism class group and the key function microorganism in the rice field soil is mined, and the method has important significance on knowing the antimony reduction process driven by the microorganism and cognizing antimony reduction bacteria and the key function gene.

Description

technical field [0001] The invention belongs to the field of soil microbial ecology, and specifically relates to a method for discriminating bacteria species and their key functional genes involved in the antimony reduction process in soil by using a stable isotope tracer-metagenomics-single bacteria sketch assembly combined platform. Background technique [0002] Antimony (Sb) is a toxic metal belonging to group 15 of the periodic table. It occurs frequently in the lithosphere of sulfur-bearing minerals, is the ninth most mined metal, and is widely used in manufacturing. The harm caused by antimony has gradually been paid attention to. For example, antimony has adverse health effects such as liver and kidney damage, pneumoconiosis, diarrhea, and dermatitis on the human body, and it is more likely to be a potential carcinogen. China is the largest antimony producer in the world, and extensive mining activities make antimony pollution an environmental problem that needs to b...

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Problems solved by technology

[0006] The defect of the above-mentioned patent is that the invention utilizes marked carbon for direct identification, which is limited to the identification of bacteria involved in the assimilation of organic matter (such as formic acid, acetic acid, organic pollutants, etc.), but heavy (like) metal compounds do not contain carbon, so Alienated respiration bacteria involved in the transformation of heavy (like) metals cannot be identified

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