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Method for quickly detecting cattle MLLT10 gene CNV marker and application of method

A cattle and gene technology, applied in the field of molecular genetics, can solve unseen problems, achieve the effects of low cost, easy popularization and application, and speed up the molecular marker-assisted selection breeding of cattle

Active Publication Date: 2019-06-28
NORTHWEST A & F UNIV
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Problems solved by technology

However, there is no report on real-time quantitative PCR for detecting the copy number variation of cattle MLLT10 gene

Method used

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  • Method for quickly detecting cattle MLLT10 gene CNV marker and application of method
  • Method for quickly detecting cattle MLLT10 gene CNV marker and application of method
  • Method for quickly detecting cattle MLLT10 gene CNV marker and application of method

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Embodiment Construction

[0028] The present invention will be described in detail below in conjunction with the accompanying drawings and embodiments, which are only explanations of the present invention, rather than limiting the protection scope of the present invention.

[0029] In the previous cattle genome resequencing study, a copy number variation located in the Chr13:23206001-23210800 region of the non-coding region of the bovine MLLT10 gene was found. Therefore, the present invention designs specific primers based on the region of copy number variation in the cattle MLLT10 gene sequence obtained by resequencing, and then uses the genomic DNA of cattle (Qinchuan cattle, Xianan cattle, Jiaxian cattle, Yunling cattle) as templates, qPCR amplification was carried out, and the BTF3 gene was used as an internal reference gene, using 2 -△△Ct A method for determining the type of copy number variation of an individual. Based on the physiological function of MLLT10 gene and the regulation mechanism of ...

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Abstract

The invention discloses a method for quickly detecting a cattle MLLT10 gene CNV marker and application of the method. Based on the real-time fluorescent quantitation PCR technology, cattle genome DNAserves as a template, the cattle MLLT10 gene copy number variation area is amplified by using specific PCR primers, and partial fragments of the cattle BTF3 gene are amplified to serve as an internalreference, and finally, by using the 2-delta delta Ct method, the individual copy number variation type is computed and determined. The method lays a basis for the relationship between the cattle MLLT10 gene copy number variation and the growth trait, the detection method is simple and fast, the method can be used for accelerating cattle marker-assisted selection breeding work, and application andpopularization are facilitated.

Description

technical field [0001] The invention belongs to the field of molecular genetics, and in particular relates to a method for detecting CNV markers of cattle MLLT10 gene. The method utilizes real-time fluorescent quantitative PCR (qPCR) technology, takes BTF3 gene as a reference, and determines individual MLLT10 gene copy number variation according to -ΔΔCt value type. Background technique [0002] In marker-assisted selection, either it is known that certain QTLs (main effect genes) exist for the traits to be evaluated, and their genotypes can be directly determined (such as QTLs discovered through candidate gene analysis), or they cannot They are genotyped, but knowing their linkage to certain markers (such as QTLs discovered by marker-QTL linkage analysis), these markers can be genotyped, at which point this information can be used for the genetic assessment of individuals , to improve accuracy. [0003] Copy number variation (CNV) is a common genetic polymorphism, mainly ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/6888C12Q1/6851
Inventor 黄永震杨鹏贺花张子敬王献伟王二耀茹宝瑞徐泽君雷初朝陈宏胡沈荣
Owner NORTHWEST A & F UNIV
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