Breeding method for corn male sterile line

A male sterile line and male sterile technology, applied in the field of maize male sterile line breeding, can solve the problems of long transfer cycle and achieve the effect of accelerating the transfer speed and stabilizing the sterility performance

Active Publication Date: 2015-01-21
BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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  • Abstract
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  • Application Information

AI Technical Summary

Problems solved by technology

However, only using the traditional backcrossing transfer method usually requires backcrossing for 5-6 generations, and the transfer cycle is longer, which delays the application of sterile seed production technology in corn hybrids

Method used

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  • Breeding method for corn male sterile line
  • Breeding method for corn male sterile line
  • Breeding method for corn male sterile line

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Embodiment 1, the breeding of male sterile line S Jing 724

[0033] 1. Determination of the type of male sterile line donor MD32 cytoplasmic male sterility

[0034] 1. Maize male sterile line MD32

[0035] Breeding process of maize male sterile line MD32: Use the constructed X line population (based on X1132) to carry out "tall and strict" line selection, and select excellent S5 high-generation lines and introduced hybrids to build a new basic line selection population. Sterile plants were found in the selfed offspring, the anthers of the tassels were not exposed, and there was no pollen. The sister plants with similar phenotypes in the same panicle row were selected for pair pollination. The hybrid offspring all showed complete sterility, and the plant phenotype tended to be basically the same. Then the material was named maize male sterile line MD32.

[0036] The maize sterile line MD32 is completely sterile: no anthers exposed, no pollen;

[0037] The sterility o...

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Abstract

The invention discloses a breeding method for a corn male sterile line. A male sterile line of corn selfing line is obtained by backcross breeding with corn S-type cytoplasmic male sterile line MD32CGMCC No.8657 as a donor and the corn selfing line as an acceptor. Experiments demonstrate that the breeding method provided by the invention has the following advantages that (1) by combining a marker-assisted selection technology and the backcross breeding technology, the breeding speed of the sterile line is accelerated and Jing 724 sterile line S-jing 724 can be obtained only by three backcross generations; and (2) the S-type sterile line MD32 selected by the breeding method presents stable and thorough sterility in a plurality of genetic backgrounds and is not used in reported studies on corn hybrid male sterile breeding.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to a method for breeding maize male sterile lines. Background technique [0002] Breeding and promotion of strong dominant hybrids is an important way to increase maize yield. The use of conventional seed production methods to prepare hybrids requires manual detasseling of the female parent, which not only consumes a lot of labor, increases the cost of seed production, but also has the potential risk of affecting seed quality due to untimely and incomplete detasseling. The use of male sterile lines for seed production is an effective method to ensure seed purity and increase maize yield. [0003] As early as the 1950s and 1960s, research on maize sterile seed production began at home and abroad. Cytoplasmic male sterility is the main way to use in maize breeding because it is easy to realize the three-line matching of sterile line, maintainer line and restorer line. Cytoplasmic male ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H1/02A01H1/04
Inventor 赵久然宋伟邢锦丰王元东王凤格段民孝李瑞媛
Owner BEIJING ACADEMY OF AGRICULTURE & FORESTRY SCIENCES
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