A detection method and application of boar star gene 5-bp repeat deletion polymorphism

Abstract

The invention discloses a method for detecting the 5-bp repeat deletion polymorphism of the boar StAR gene and its application. This method uses the whole genome DNA of the boar to be tested as a template, amplifies the StAR gene fragment by PCR technology, and then performs agarose gel electrophoresis. According to the electrophoresis results, it is identified that the StAR gene exists at the NC_010457.5:g.5524‑5528 site5 ‑bp repeat deletion polymorphism. The different genotypes of StAR gene 5-bp repeat deletion polymorphism were significantly correlated with reproductive traits such as testicular long axis length, testicular short axis length and testicular weight in 15-day-old and 40-day-old Large White boars. The method for detecting the insertion / deletion of the boar StAR gene provided by the invention can be applied in the marker-assisted selection breeding of the reproductive traits of the boar, and is beneficial to quickly establish a boar genetic resource population with excellent reproductive performance.

Description

technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to the detection of gene insertion / deletion (indel), in particular to a method for rapidly and accurately detecting boar StAR gene NC_010457.5:g.5524-5528 site 5-bp repeat deletion multiple method and its application. Background technique [0002] With the improvement of human living standards, the consumer demand for livestock, especially pigs (Sus scrofa), is getting higher and higher, and the pig breeding industry has developed rapidly. China is a big pork consumer, and pork accounts for more than 60% of the total meat consumption. According to relevant statistics, in China, the annual per capita pork consumption is about 59 kilograms, which means that Chinese consumers account for more than half of the world's pork consumption. Therefore, cultivating germplasm resources with excellent traits is the guarantee for sufficient pork supply. [0003] With the devel...

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Problems solved by technology

[0010] At present, the research on StAR gene mainly focuses on human-related diseases, but the research on the association between the genetic variation of StAR gene and the reproductive traits of boars is very scarce.

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