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Detection method for 4 repeated deletion polymorphism sites of Chinese yellow cattle PPP2R2B gene and application of method

A polymorphic locus and gene locus technology, which is applied in the determination/inspection of microorganisms, biochemical equipment and methods, etc., can solve problems such as affecting the growth traits of cattle, and achieve low-cost effects.

Active Publication Date: 2019-04-16
NORTHWEST A & F UNIV
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Problems solved by technology

Therefore, indels in the base sequence of the PPP2R2B gene may affect the growth traits of cattle

Method used

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  • Detection method for 4 repeated deletion polymorphism sites of Chinese yellow cattle PPP2R2B gene and application of method
  • Detection method for 4 repeated deletion polymorphism sites of Chinese yellow cattle PPP2R2B gene and application of method
  • Detection method for 4 repeated deletion polymorphism sites of Chinese yellow cattle PPP2R2B gene and application of method

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Embodiment Construction

[0072] This example provides four kinds of Chinese yellow cattle whole genome DNA as templates, using primer pairs P1-P4 to amplify partial fragments of Chinese yellow cattle PPP2R2B gene by PCR; then perform agarose gel electrophoresis on the PCR amplified fragments; according to electrophoresis The results identified the genotypes of the insertion / deletion polymorphism sites in the PPP2R2B gene of Chinese yellow cattle; among them:

[0073] The primer pair P1 is:

[0074] Upstream primer: 5'-GCATCAGCCCTAAATCCCA-3';

[0075] Downstream primer: 5'-CAGAAAATATCAAAATGCCAGAA-3'.

[0076] The primer pair P2 is:

[0077] Upstream primer: 5'-AGTCTTAGTTTAGAGGGCTTCC-3';

[0078] Downstream primer: 5'-GCTTAGTCATTCAGCCATTTT-3'.

[0079] The primer pair P3 is:

[0080] Upstream primer: 5'-GCTGATCCCTCAAATATGGC-3';

[0081] Downstream primer: 5'-TGAAAGCGTCAAGAGCAAG-3'.

[0082] The primer pair P4 is:

[0083] Upstream primer: 5'-CAACTGGAAAAGCTAAGGC-3';

[0084] Downstream primer: 5'-TGGAATCAGATGGAAGAAAG-3'...

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Abstract

The invention discloses a detection method for 4 repeated deletion polymorphism sites of a Chinese yellow cattle PPP2R2B gene and application of the method. According to the method, Chinese yellow cattle full-genome DNA is taken as a template, and a Chinese yellow cattle PPP2R2B gene partial fragment is amplified through PCR by using a primer pair P1-P4; agarose gel electrophoresis is performed onthe fragment obtained through PCR amplification; the genotype of an insertion / deletion polymorphism site on the Chinese yellow cattle PPP2R2B gene is identified according to an electrophoresis result; the different genotypes of the repeated deletion polymorphism of the gene are obviously related to the growth traits such as body height, body length, chest circumference, chest breadth, chest depthand waist height of four Chinese yellow cattle comprising Qinchuan cattle, Nanyang cattle, Jiaxian cattle and Luxi cattle. The method can be applied to marker-assisted selection breeding of beef cattle growth traits, and is conductive to rapidly establishing a Chinese yellow cattle population with excellent genetic resources, thereby accelerating the selective breeding speed of the Chinese yellowcattle with the excellent growth traits.

Description

Technical field [0001] The invention belongs to the field of biotechnology and livestock breeding, and relates to a method for detecting indels of genes, and in particular to a method for detecting four duplication and deletion polymorphism sites in the PPP2R2B gene of yellow cattle. The method can quickly and accurately detect Chinese cattle PPP2R2B gene AC_000164.1 locus 4 repeat deletion polymorphisms, and can be used in the application of Chinese yellow cattle molecular marker assisted selection breeding. Background technique [0002] With the rapid development of China's economy today, people's lives are entering a well-off society. The demand for meat, eggs, milk and other animal-derived foods is increasing, and the requirements for variety and quality are increasing. Although the proportion of the total output value of the animal husbandry industry has exceeded one third of the total agricultural output value, the overall production level of the animal husbandry industry i...

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Application Information

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IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王淑辉孙秀柱李泽樊路杰王国艳江钧益刘世荣易晓华
Owner NORTHWEST A & F UNIV
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