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Detection method and application of 4 duplication-deletion polymorphic sites of ppp2r2b gene in Chinese yellow cattle

A polymorphic locus, gene locus technology, applied in the direction of biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as affecting cattle growth traits, and achieve low-cost effects

Active Publication Date: 2021-11-12
NORTHWEST A & F UNIV
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Problems solved by technology

Therefore, indels in the base sequence of the PPP2R2B gene may affect the growth traits of cattle

Method used

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  • Detection method and application of 4 duplication-deletion polymorphic sites of ppp2r2b gene in Chinese yellow cattle
  • Detection method and application of 4 duplication-deletion polymorphic sites of ppp2r2b gene in Chinese yellow cattle
  • Detection method and application of 4 duplication-deletion polymorphic sites of ppp2r2b gene in Chinese yellow cattle

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Embodiment Construction

[0072] The present embodiment provides four kinds of using the whole genome DNA of Chinese yellow cattle as a template, using primer pairs P1-P4 to amplify partial fragments of the PPP2R2B gene of Chinese yellow cattle by PCR; The results identified the genotype of the insertion / deletion polymorphic site on the PPP2R2B gene of Chinese yellow cattle; wherein:

[0073] Primer pair P1 is:

[0074] Upstream primer: 5'-GCATCAGCCCTAAATCCCA-3';

[0075] Downstream primer: 5'-CAGAAAATATCAAAATGCCAGAA-3'.

[0076] Primer pair P2 is:

[0077] Upstream primer: 5'-AGTCTTAGTTTAGAGGGCTTCC-3';

[0078] Downstream primer: 5'-GCTTAGTCATTCAGCCATTTT-3'.

[0079] Primer pair P3 is:

[0080] Upstream primer: 5'-GCTGATCCCTCAAATATGGC-3';

[0081] Downstream primer: 5'-TGAAAGCGTCAAGAGCAAG-3'.

[0082] Primer pair P4 is:

[0083] Upstream primer: 5'-CAACTGGAAAAGCTAAGGC-3';

[0084] Downstream primer: 5'-TGGAATCAGATGGAAGAAAG-3'.

[0085] The insertion / deletion polymorphic site on the Chinese y...

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Abstract

The invention discloses a detection method and application of four repeat-deletion polymorphic sites of the PPP2R2B gene of Chinese yellow cattle. The method uses the whole genome DNA of Chinese yellow cattle as a template, uses the primer pair P1‑P4 to amplify a partial fragment of the PPP2R2B gene of Chinese yellow cattle by PCR; then performs agarose gel electrophoresis on the fragments obtained by PCR amplification; and identifies the PPP2R2B of Chinese yellow cattle according to the electrophoresis results The genotype of the insertion / deletion polymorphism site on the gene; the different genotypes of the duplication and deletion polymorphism of the gene are respectively related to the body height of four Chinese yellow cattle, including Qinchuan cattle, Nanyang cattle, Jiaxian cattle and Luxi cattle , body length, bust girth, chest width, chest depth, waist height and other growth traits were significantly correlated. The method can be applied in marker-assisted selection breeding of beef cattle growth traits, and is conducive to the rapid establishment of Chinese cattle populations with excellent genetic resources, thereby speeding up the breeding of Chinese cattle with excellent growth traits.

Description

technical field [0001] The invention belongs to the fields of biotechnology and livestock breeding, and relates to a detection method for gene insertion and deletion (indel), in particular to a detection method for four repeat-deletion polymorphic sites in the PPP2R2B gene of cattle, which can quickly and accurately detect Chinese cattle PPP2R2B gene AC_000164.1 site 4 repeat deletion polymorphisms, and can be used in the application of Chinese yellow cattle molecular marker-assisted selection breeding. Background technique [0002] Today, China's economy is developing rapidly, and people's lives are entering a well-off society. The demand for animal-derived foods such as meat, eggs, and milk is increasing, and the requirements for variety and quality are increasing. Although the proportion of the total output value of animal husbandry has exceeded one-third of the total agricultural output value, the overall production level of animal husbandry is still relatively low. As ...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6888
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王淑辉孙秀柱李泽樊路杰王国艳江钧益刘世荣易晓华
Owner NORTHWEST A & F UNIV
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