Establishment of dual-SSR-PCR system for polymeric gene 7198 of muskmelon

Abstract

The invention relates to establishment of a dual-SSR-PCR system for a polymeric gene 7198 of a muskmelon and belongs to the field of biotechnology breeding and the dual-SSR-PCR system is specially used for polymeric breeding of gummy-stem-blight-resistant genes Gsb-1 and Gsb-4 of the muskmelon. By simultaneously adding primers CMCT505 and CMTA170a and adjusting concentrations of DNTPs, Buffer and a template DNA, an annealing temperature and the like, orthogonal tests of various affecting factors are designed, by identifying DNA of polymeric gene seedlings obtained by amplifying and screening bi-parental progeny F1 and a single PCR system, the dual-SSR-PCR system capable of simultaneously amplifying 189bp and 121bp two target strips is established. The dual-SSR-PCR system has the advantages that the time is saved, the cost is reduced and the efficiency is improved, especially valuable experimental materials are saved and important reference significance for practices and theoretical research on polymeric breeding of the muskmelon and whole vegetable crops is realized.

Description

1. Technical field [0001] The establishment of the double SSR-PCR system of the muskmelon polymer gene 7198 belongs to the field of biotechnology breeding, and relates to the rapid molecular marker screening of a polymer gene material resistant to vine blight. 2. Technical background [0002] Hami melon (Cucumis melo var. saccharins) is an important economic crop in Xinjiang. It is sweet and juicy, delicate in taste, crispy in meat and rich in nutrition. It is considered as the best fruit and enjoys a high reputation at home and abroad. Traditional cantaloupe is only suitable for growing in the arid and semi-arid desert regions of Xinjiang, but the area for planting cantaloupe in this area is small, which is far from meeting the demand. Due to consumer demand for cantaloupes, cantaloupes have been planted nationwide. At present, there are still some problems that need to be solved urgently in the production of Hami melon in Xinjiang and the whole country. Among them, the oc...

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Problems solved by technology

[0007] The present invention aims at the present situation that the efficiency of step-by-step identification method for the molecular marker of muskmelon resistance to vine blight 7198 is relatively low, the test cost is relatively high, and it is easy to cause waste of valuable experimental materials, etc., and provides a dual method established by using primers CMCT505 and CMTA170a. The SSR-PCR system can quickly identify disease-resistant single plants containing the aggregated gene 7198 at one time, which will greatly accelerate the screening and identification of aggregated resistance sources of muskmelon resistance to vine blight, and reduce the consumption of test costs

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