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KASP marker relative to wheat root system character and application of KASP marker

A wheat and root technology, applied in recombinant DNA technology, microbial determination/inspection, DNA/RNA fragments, etc., can solve the problem of rarely closely linked markers, etc.

Active Publication Date: 2018-05-22
INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Most genes for root traits are associated with genes related to fertilizer and water use, agronomic traits, and yield traits (Bai et al., 2013), but few closely linked markers have been reported

Method used

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  • KASP marker relative to wheat root system character and application of KASP marker
  • KASP marker relative to wheat root system character and application of KASP marker
  • KASP marker relative to wheat root system character and application of KASP marker

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Example 1. Obtaining of special primers for KASP markers related to wheat root traits

[0049] 1. Investigation of root traits and analysis of SNP markers

[0050] 1. Investigation of root traits

[0051] Yangmai 16 (China Agricultural Development Group Co., Ltd., variety right number: CNA20030436.4) / Zhongmai 895 (Henan Xinda Agricultural Development Co., Ltd., National Examination Mai 2012010) DH population, including parents, a total of 200 families were selected as experimental materials Conduct root tests to investigate root traits.

[0052] The root test adopts the hydroponic method, that is, 30 seeds are selected for each experimental material. The specific steps are as follows: use 10% H 2 o 2 Soak the treated seeds for 15-20 minutes, rinse them with sterile water 5-6 times; then select seeds that are full and of the same size from the treated seeds and place them in a petri dish covered with filter paper, and germinate them in the darkroom of the incubator; ...

Embodiment 2

[0070] Embodiment 2, the application of SNP

[0071] 24 offspring varieties of Feiyangmai 16 / Zhongmai 895DH population and Yangmai 16 were selected. Among them, 24 offspring varieties of non-Yangmai 16 / Zhongmai 895DH population were the test group, and Yangmai 16 was the control group; including Huai Mai 20, Jimai 19, Jimai 21, Jingshuang 16, Jimai 22, Jinmai 45, Liangxing 66, Zhoumai 20, Zhengnong 21, Luomai 26, Zhongjin 13, Mianyang 26, Yannong 19, Wanmai 38, Wennong 14, Xiaoyan 54, Guangtai 68, Xianmai 12, Yumai 63, Zhoumai 16, Zhoumai 18, Zhoumai 22, Zhoumai 31 and Zhoumai 32.

[0072] 1. Detection of root traits of different varieties of wheat at seedling stage

[0073] The identification of root traits of wheat varieties at the seedling stage was carried out in the greenhouse of the Institute of Crop Science, Chinese Academy of Agricultural Sciences in the winter of 2016. Choose 30 seeds that are full and of the same size for each variety, and use 10% H 2 o 2 Treat ...

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Abstract

The invention discloses a KASP marker relative to the wheat root system character and application of the KASP marker. A special primer for the KASP marker can be used for identifying whether a genotype of wheat to be tested is TT, CC or CT and can determine the superficial area of the root system according to the genotype of the wheat to be tested; and the superficial area of the root system of the TT-genotype wheat to be tested is larger than the superficial area of the root system of CT or CC-genotype wheat to be tested, so that the KASP marker can be used for screening wheat varieties withexcellent root system character, lays the theoretical foundation for breeding the wheat varieties with high and stable yield and good quality and provides a marker assisted selection means.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to KASP markers related to wheat root traits and applications thereof. Background technique [0002] Excellent root morphology and rhizosphere processes are the basis for efficient plant nutrient utilization (Kaushik et al., 2017), and play a key supporting role in promoting crop growth and yield formation. Due to the excessive use of nitrogen fertilizers in production, a series of waste of resources and environmental problems have been caused (Chuan et al., 2015). Breeders try to improve the efficiency of fertilizer use by crops to meet the dual needs of increasing yield and being environmentally friendly. Root development in the initial stage of plant growth is extremely sensitive to nutrient application. Using genetic breeding methods to master root systems with long-lasting and stable vitality that is compatible with the growth and development of aboveground parts has become an impo...

Claims

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Application Information

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IPC IPC(8): C12Q1/6895C12Q1/6858C12N15/11
CPCC12Q1/6858C12Q1/6895C12Q2600/13C12Q2600/156C12Q2600/172C12Q2563/107C12Q2531/113
Inventor 何中虎杨梦娇王彩荣肖永贵夏先春
Owner INST OF CROP SCI CHINESE ACAD OF AGRI SCI
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