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Molecular marker relevant to chicken growth traits, as well as identification method and application thereof

A technology for growth traits and molecular markers, which is applied in biochemical equipment and methods, microbiological measurement/inspection, DNA/RNA fragments, etc. It can solve the problems of unseen application and establishment of BMP7 gene, etc., achieving high efficiency and low cost , high accuracy effect

Active Publication Date: 2016-11-09
ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, from a genetic point of view, there is no established detection method for the BMP7 gene as a molecular marker for chicken growth traits, and at the same time, there is no application of this gene as a molecular marker for chicken growth traits.

Method used

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  • Molecular marker relevant to chicken growth traits, as well as identification method and application thereof
  • Molecular marker relevant to chicken growth traits, as well as identification method and application thereof
  • Molecular marker relevant to chicken growth traits, as well as identification method and application thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0025] Embodiment 1: the establishment of chicken growth character molecular detection method

[0026] (1) Primers:

[0027] Taking the DNA sequence of the chicken BMP7 gene (the reference sequence GeneBank accession number is NC_006107.3) as a template, a pair of primers BMP7-F / BMP7-R were designed and cloned to obtain the DNA sequence shown in sequence SEQ ID NO: 1 (including part of the 6 intron and part of the 7th exon), the full length of the sequence is 1352bp. The primer sequences used are respectively shown in SEQ ID NO: 2 and SEQ ID NO: 3, specifically as follows:

[0028] BMP7-F: 5'-TCCTCTGATTGCTTTACTGACTTG-3',

[0029] BMP7-R: 5'-GGGAAGGAGGTATCATTGGAGA-3'.

[0030] (2) PCR amplification conditions:

[0031] Add 1 μL of DNA template, 12.5 μL of 2×PCR reaction mixture, 1 μL of 10 mM primer before and after, 0.625 U of Taq DNA polymerase, and 8.25 μL of double distilled water into the PCR reaction system with a total volume of 25 μL. The PCR reaction conditions are...

Embodiment 2

[0042] Embodiment 2: Detection and application of the distribution of PCR-EcoRI-RFLP polymorphism in various chicken breeds

[0043] (1) Detection of the distribution of PCR-EcoRI-RFLP polymorphism in various chicken breeds

[0044] In this case, the blood DNA of 8 breeds of chickens was collected, and the samples were all from the original chicken farm of the Institute of Animal Science, Guangdong Academy of Agricultural Sciences. According to the method described in Example 1, the PCR-EcoRI-RFLP detection was carried out to the above chicken species. The allele frequency of the mutation site in different chicken species is shown in Table 1. The T allele is dominant in the long Lingnan yellow chicken A line and the recessive White Locke breed, while the C allele is dominant in the red Jungle chicken, Huiyang bearded chicken and Xinghua chicken breed. The proportions of T and C alleles in Xiayan Chicken and Beijing You Chicken are similar. Table 1 shows the genotype and alle...

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Abstract

The invention discloses a molecular marker relevant to chicken growth traits, as well as an identification method and application thereof. The molecular marker is C / T base mutation at the 994th base in the sequence shown as SEQ ID NO:1. The molecular marker takes a DNA sequence of chicken BMP7 gene as a template, and selects primers shown as SEQ ID NO:2 and SEQ ID NO:3 for amplification. Through the detection of the C / T mutation site, the genotype of relevant growth traits of a target chicken flock can be known, the chicken flock can be selected on purpose, and the consistencies of weight, shin length and shin girth of a descendant chicken flock can be improved. In the breeding process, TT genotype individuals are selected, and chicken growth indexes such as weight, shin length, shin girth and the like can be improved. Moreover, the identification method used in the invention is high in accuracy, low in cost and high in efficiency.

Description

technical field [0001] The invention relates to the technical field of chicken marker-assisted selection, in particular to a molecular marker related to chicken growth traits and its identification method and application. Background technique [0002] In the past 60 years, the growth rate and meat yield of broilers have been greatly improved, but the improvement of growth traits has also brought some problems, such as obesity, ascites, and leg diseases. Leg diseases will lead to reduced feed intake, growth inhibition, decreased immunity and carcass quality, and lower production performance will directly increase breeding costs. Enhancing bone strength and maintaining bone proportions has become an important goal in current broiler production as a way to prevent leg problems. Studies have reported that leg problems in broilers are influenced by feeding methods, genetics, etc. The BMP family was discovered and named as bone morphogenetic protein (Bone Morphogenetic Protein, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68C12N15/11
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 王艳郭福有刘天飞瞿浩罗成龙舒鼎铭王劼
Owner ANIMAL SCI RES INST GUANGDONG ACADEMY OF AGRI SCI
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