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Sheep lambing number trait related molecular marker and application thereof

A technology of molecular markers and litter size, which is applied in the determination/inspection of microorganisms, DNA/RNA fragments, recombinant DNA technology, etc., can solve problems such as the impact of livestock reproductive performance, loss of receptor function, and reduced expression of LHβ

Active Publication Date: 2014-10-15
甘肃润牧生物工程有限责任公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The function of LHβ must depend on its receptors. Mutations in LHβ or LHR genes may lead to decreased expression of LHβ, inactivation or partial inactivation of the active site binding to receptors, or reduction of LHR or loss of receptor function. Both will lead to G protein coupling and signal transmission obstacles, and then affect the reproductive performance of livestock (Zhang Xueqin et al. Research progress on LH / CG receptors [J]. Grass Industry and Animal Husbandry. 2008,4:1-9.)

Method used

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  • Sheep lambing number trait related molecular marker and application thereof
  • Sheep lambing number trait related molecular marker and application thereof
  • Sheep lambing number trait related molecular marker and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0033] Example 1: The acquisition of gene fragments and the establishment of polymorphism detection methods

[0034] Amplification of Sheep LHβ Gene Sequence

[0035] (1) Collect blood samples

[0036] Select one foreign sheep breed (Dorper, South African Meat Merino sheep blood sample collected from Minqin Zhongtian Sheep Industry Co., Ltd., which is a conventional breed) and two local Chinese sheep breeds (Hu sheep, Small-tailed Han sheep, blood sample collected from Jinchang Zhongtian Sheep Industry Co., Ltd., which is a conventional variety) was used as the test material, and the whole blood of sheep was taken;

[0037] (2) Genomic DNA extraction

[0038] Genomic DNA was extracted from the whole blood of sheep. The DNA samples were all extracted using the Genomic DNA Kit (TIANamp Genomic DNA Kit) produced by Beijing Tiangen Biochemical Technology Co., Ltd., and were operated and extracted according to the operating instructions provided by the kit.

[0039] (3) Primer d...

Embodiment 2

[0047] Example 2: Application of molecular markers prepared by the present invention in association analysis of sheep litter size-related traits

[0048] A total of 18 Dorper sheep, 270 Hu sheep, 869 Small-tailed Han sheep, 5 South African meat Merino sheep, 5 Duhan F1 generation (♂Dorper sheep×Small-tailed Han sheep♀), 106 The polymorphism of the F1 generation of Duhu sheep (♂ Dorbo sheep × Hu sheep ♀) and 145 F1 generations of Meihu sheep (♂ South African meat Merino sheep × Hu sheep ♀), the genotypes were determined, and the genetic Association analysis of type and litter size.

[0049] Build a least squares model as follows:

[0050] Y ijkl =μ+Genotype i +Breed j +P k +S l +Combination m +ε ijklm .

[0051] Among them, Y ijkl is the observed value of traits, μ is the overall mean, Genotype i is the genotype effect, Breed j is the variety effect, P k is the parity effect, S i For the seasonal effect, Combination l is the combined effect, ε ijkl is a random e...

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Abstract

The invention belongs to the technical field of preparation of molecular markers for livestock, and particularly relates to a preparation method of a sheep lambing number trait related molecular marker serving as MAS (marker-assisted selection) as well as an application of the molecular marker. The molecular marker is obtained through LH (luteinizing hormone) beta gene cloning, and the nucleotide sequence is represented in SEQ ID NO.1. One C713-T713 base is substituted at 713 bp in the SEQ ID NO.1, so that BSrB I-RFLP (restricted fragment length polymorphisms) is caused. The invention further discloses a primer used in a DNA (deoxyribonucleic acid) sequence of an amplified LH beta gene part and a polymorphism detection method, and provides one novel molecular marker for MAS of the sheep lambing number trait.

Description

technical field [0001] The invention relates to the technical field of preparation of sheep molecular markers, in particular to a molecular marker related to sheep litter size traits and its application. The molecular marker is cloned from the sheep LHβ gene, which includes the whole genome sequence mutation of the sheep LHβ gene Site detection methods and applications. Background technique [0002] The reproductive traits of sheep are important economic traits, and the litter size is one of the main reproductive traits. Studies have shown that the reproductive traits of sheep belong to the threshold traits of low heritability, and the number of litters (lambs) of animals is an important factor affecting production efficiency. It is controlled by genes, and it is an additive-dominant gene action mode (Chu Xingxing et al. . Major gene and its application in animal breeding [J]. Journal of Agricultural Biotechnology, 1995, 3 (4): 23-31.). The selected target trait of the pre...

Claims

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Application Information

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IPC IPC(8): C12N15/11C12Q1/68
Inventor 李发弟王维民潘香羽刘世佳翁秀秀马友记唐德富李冲
Owner 甘肃润牧生物工程有限责任公司
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