Method for detecting CNV (copy number variation) mark of MAPK10 gene of Nanyang cattle and application thereof

A gene and marker technology, applied in biochemical equipment and methods, microbial determination/inspection, etc., can solve problems such as the influence of Nanyang cattle growth traits and gene function that have not been seen, and achieve the effect of simple operation and accurate and reliable method.

Active Publication Date: 2016-04-20
NORTHWEST A & F UNIV
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Problems solved by technology

[0007] Resequencing the whole genome of Nanyang cattle revealed a 1776bp copy number variation (CNV) at the 10th intron of the MAPK10 gene, which may affect the function of the gene
At present, there is no literature report on the effect of this CNV of MAPK10 gene on the growth traits of Nanyang cattle

Method used

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  • Method for detecting CNV (copy number variation) mark of MAPK10 gene of Nanyang cattle and application thereof
  • Method for detecting CNV (copy number variation) mark of MAPK10 gene of Nanyang cattle and application thereof
  • Method for detecting CNV (copy number variation) mark of MAPK10 gene of Nanyang cattle and application thereof

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Embodiment Construction

[0032] The present invention utilizes QPCR to detect the copy number variation of the MAPK10 gene of Nanyang cattle. The present invention will be described in detail below in conjunction with the accompanying drawings and examples, which are explanations rather than limitations of the present invention.

[0033] A CNV marker detected based on QPCR technology involved in the present invention refers to the copy number variation of the bovine MAPK10 gene candidate region (Chr6: 102708523-102710298). Specifically, the CNV marker is located in the 610515bp-612290bp region of the sequence shown in the bovine MAPK10 gene (GenBankAccessionNo.AC_000163), according to the log 2 2 -ΔΔCt Divide the results into three categories: Log 2 2 -ΔΔCt >0.5 is plug-in type; Log 2 2 -ΔΔCt 2 2 -ΔΔCt ≤|±0.5| is the normal type.

[0034] 1. Nanyang Cattle Sample Collection

[0035] In the present invention, the local Nanyang cattle breed in China is used as the detection object, and blood samp...

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Abstract

The invention discloses a method for detecting a CNV (copy number variation) mark of a MAPK10 gene of Nanyang cattle and an application thereof. The method comprises the following steps: respectively amplifying the CNV region and reference gene BTF3 of the MAPK10 gene of Nanyang cattle through a real-time fluorescence quantification PCR method by taking blood genome DNA of Nanyang cattle as a template; dividing the result into insert type, deficient type and normal type according to log22-delta delta Ct; and identifying CNV of the MAPK10 gene of Nanyang cattle. Analysis correlated to Nanyang cattle production data indicates that the growth and development of Nanyang cattle is remarkably influenced by different copy numbers of MAPK10, wherein the birth weight, 18-month-aged body height and 24-month-aged body weight and daily gain of a normal type individual body are remarkably higher than those of the insert type and deficient individual bodies. The CNV mark closely related to the production traits of Nanyang cattle is detected on DNA level, and can be used as an important candidate molecular mark for marker assisted selection of the growth traits of Nanyang cattle of China.

Description

technical field [0001] The invention relates to the field of livestock molecular biology detection, in particular to a method for detecting CNV markers of Nanyang cattle MAPK10 gene based on QPCR technology. Background technique [0002] With the rapid development of genomics and bioinformatics, animal breeding theory and technology are undergoing major changes, and the direction of beef cattle breeding is developing from conventional phenotypic breeding to molecular breeding. At present, the research on molecular breeding of cattle mainly focuses on marker-assisted selection based on molecular markers. Molecular breeding, that is, molecular marker-assisted selection (MAS), is a technology that uses DNA molecular markers to select genetic resources or breeding materials, and to improve the comprehensive traits of livestock and poultry. In livestock and poultry breeding, through the selection of DNA markers that are closely related to growth traits and quantitative traits, t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/68
CPCC12Q1/6888C12Q2600/124C12Q2600/156
Inventor 陈宏刘梅白跃宇李波杨明娟蓝贤勇黄永震
Owner NORTHWEST A & F UNIV
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