Screening and application of fluorescent quantitative reference genes of pear fruits at different development stages

Abstract

The invention discloses screening and application of fluorescent quantitative reference genes of pear fruits at different development stages, and relates to the field of pear molecular biology researches. Reference genes, which are more stable than common housekeeping genes at different development stages of Cuiguan, Fengshui and Xueqing pear fruits, are pertinently obtained according to dynamic RNA (Ribonucleic Acid)-Seq sequencing results, post-period experience validation and data analysis of pear fruits of various varieties; the difference between samples and the difference in the samples, which are caused by the selection of the reference genes, are reduced to the greatest extent; the correction and standardization of a target gene expression level can be realized, and accurate quantification of functional gene expression of the pear fruits is realized.

Description

technical field [0001] The invention relates to the field of molecular biology research, in particular to the screening and application of real-time fluorescent quantitative PCR internal reference genes during pear fruit development. Background technique [0002] Pear (Pyrus) is one of the main fruit trees in my country. It has a long history of cultivation. The cultivation area covers all parts of the country. The cultivation area and output rank first in the world. In recent years, Chinese researchers have carried out many studies on pears from the molecular biology level, especially after the completion of the world's first pear whole genome sequencing, assembly and gene annotation in May 2012, gene expression analysis has been widely used to reveal the pear fruit. In the study of gene regulation mechanism. [0003] Real-time fluorescent quantitative PCR has the characteristics of high sensitivity, good repeatability, strong specificity and high throughput, and is curren...

AI Technical Summary

Problems solved by technology

However, more and more reports point out that under different growth stages or experimental conditions, the results of traditional housekeeping genes used as internal reference genes by fluorescent quantitative PCR are very different, and cannot be stably expressed, sometimes leading to contrary conclusions, which cannot truly reflect gene expression level

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