Gene methylation detection method

Abstract

The invention discloses a gene methylation detection method. The method comprises the steps of: subjecting a to-be-detected DNA sample, a negative control, a positive control and a no template control to PCR amplification reaction, with the DNA sample in the PCR amplification reaction being bisulfite modified DNA, carrying out duplex PCR amplification reaction on a detection gene and a reference gene, and interpreting a parameter range to judge the methylation condition of the detection gene of the to-be-detected DNA sample. By the mode, the gene methylation detection method provided by the invention has the advantages of strong specificity, duplex reaction, high sensitivity, small pollution, simple and quick operation, and safety, etc., and the detection result has good accuracy and repeatability. Thus, the method has certain auxiliary diagnosis effect on early detection of colorectal cancer and other cancers.

Description

technical field [0001] The invention relates to the field of gene detection, in particular to a method for detecting gene methylation. Background technique [0002] Alterations in the level and pattern of DNA methylation are an important factor in tumorigenesis, and these changes include localized hypermethylation of CpG islands and hypomethylation status of genomic DNA. Since the local hypermethylation of CpG islands is earlier than the malignant proliferation of cells, the detection of its methylation can be used for the prediction of tumors, and the low-level methylation status of the whole genome level will increase with the increase of tumor malignancy. Further reduced, so that it can be used in the diagnosis and grading of tumors. Methylation can be used as a biomarker and prognosis evaluation index for early diagnosis of tumors, and is of great significance for tumor screening and risk assessment, early diagnosis, staging and typing, prognosis judgment and treatment ...

AI Technical Summary

Problems solved by technology

This is a classic methylation research method. Its advantages are: relatively simple, low cost, clear methylation sites, and easy interpretation of experimental results. The disadvantages are: 1. Since CG is not limited to CCGG sequences, it is not The CG in this sequence will be ignored; 2. Only when the methylation status of key sites related to transcription is detected, the results of this detection method are meaningful; 3. Relatively speaking, the Southern method is more complicated and requires The amount of sample is large; 4. There is a problem of false positives caused by incomplete enzyme digestion; 5. It is not suitable for mixed samples

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