Method for screening reference genes for chilo suppressalis under temperature stress and application of reference genes

A technology of internal reference gene and Chilo suppressalis, which is applied in the direction of biochemical equipment and methods, microbe determination/inspection, etc., can solve the problem of non-existence of internal reference gene
CN105274239AActive Publication Date: 2016-01-27ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
ZHEJIANG ACADEMY OF AGRICULTURE SCIENCES
Publication Date
2016-01-27

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Abstract

The invention discloses a method for screening reference genes for chilo suppressalis under different temperature stress conditions and application of the reference genes. The method includes utilizing the chilo suppressalis cultivated under room-temperature conditions as a control material; carrying out fluorescence quantitative PCR (polymerase chain reaction) detection by the aid of the chilo suppressalis as an experimental material after the chilo suppressalis is under high-temperature and low-temperature stress; inputting Ct values of all alternative reference genes into delta Ct, RefFinder, BestKeeper, GeNorm, NormFinder reference gene stability analysis software and analyzing the Ct values of all the alternative reference genes; screening the most stable in-vivo reference genes TUB for the chilo suppressalis under the temperature stress conditions. The method and the application have the advantage that expression of genes related to the temperature stress conditions can be accurately studied by the aid of genes, namely the reference genes.
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Description

technical field

[0001] The invention belongs to the technical field of expression stability of real-time fluorescent quantitative PCR internal reference genes in molecular biology, and in particular relates to a screening method and application of internal reference genes of Chilo suppressalis under different temperature stresses. Background technique

[0002] Chilosuppressalis belongs to the family Lepidoptera and is an important pest of rice in my country. It causes symptoms such as withered sheath, dead heart, white ear, withered booting ear and insect damage to rice, causing huge economic losses. With the renewal of rice cultivation system and the change of rice varieties, the population of Chilo suppressalis increases year by year.

[0003] Under low temperature conditions in winter, Chilo borer survives the winter in rice stumps, straw, and other crop residues such as grasses in the field. It begins to emerge in early May, and its damage runs through the entire growing...

Claims

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