Microsatellite marker primers for identification of Acipenser dabryanus family and identification method thereof

Abstract

The invention relates to microsatellite marker primers for identification of an Acipenser dabryanus family. Through a transcriptome sequencing technology, Unigenes of the screened Acipenser dabryanus are screened, microsatellite loci in the Unigenes sequence are detected, according to flanking sequences at two ends of each one of the microsatellite molecular marker loci, specific primers of the molecular marker are labeled, the specific primers are subjected to PCR amplification, the stability and polymorphism of the amplification result are detected, and 18 effective microsatellite markers are acquired. Through the microsatellite marker primers, according to the polymorphism of the microsatellite marker primers, a parentage identification platform of Acipenser dabryanus is built and the basis is provided for Acipenser dabryanus selection and breeding matching.

Description

technical field [0001] The invention relates to the technical field of molecular markers, in particular to a microsatellite marker primer and an identification method for the family identification of Dabry's sturgeon. Background technique [0002] Dabry's sturgeon, also known as Changjiang sturgeon and Sarba, is a unique species in my country. It is mainly distributed in the main stream of the Yangtze River and the lower reaches of the Jinsha River, and the Yibin-Hejiang section of Sichuan has a higher yield. In the past 20 years, due to the construction of Gezhouba Project, water pollution, overfishing and other reasons, the wild resources of Dabry's sturgeon have been extremely scarce. In 1988, it was listed as a national first-level key protected animal and a first-level urgently protected fish in the upper reaches of the Yangtze River. Domestication and breeding of wild resources of Dabry's sturgeon, and artificial reproduction after reaching sexual maturity are importan...

AI Technical Summary

Problems solved by technology

However, the method of cultivating a single family alone not only takes up a lot of space, but also has high labor intensity and high cost of breeding equipment. More importantly, different environmental effects will affect the growth traits of different families; and traditional physical markers are cumbersome and expensive. , Harm to the fish body, easy to fall off the mark and other disadvantages, cannot distinguish a single individual

It is very difficult to obtain reliable genetic relationship and pedigree structure information using traditional methods

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