Method and kit for screening target region of methylation PCR detection and application of target region

Abstract

The invention discloses a method and a kit for screening a target region of methylation PCR detection and an application of the target region. The method comprises the following steps: (1) acquiring ato-be-analyzed tumor methylation chip and corresponding transcriptome sequencing data from a database; (2) calculating methylation degree values of a normal group and a cancer group, and screening methylation sites, which keep obvious differences, in the various groups; (3) in combination with analysis on a transcriptome sequencing expression profile, calculating related coefficients, and screening negatively related sites; and (4) associating the methylation candidate sites which are acquired in the step (3) with related disclosed documents, screening sites which are supported and reported by many documents, keep a high inter-group methylation difference group and are in expression amount negative relation, and on the basis of a regression algorithm, acquiring a site set, namely the target region, achieving the optimal sensitivity and specificity. According to the method, through comprehensive analysis of the database, transcriptome sequencing and the documents and in combination with multiple data filtering and the regression algorithm, the target region of methylation PCR detection can be obtained sensitively and specifically.

Description

technical field [0001] The present application relates to the field of nucleic acid methylation PCR detection, in particular to a method, kit and application for screening target regions of methylation PCR detection. Background technique [0002] Abnormal DNA methylation and gene mutation are important reasons for the occurrence and development of tumors. The former may have occurred in the very early stage of tumors and is the "seed" factor that promotes tumor growth. In mammals, DNA methylation mainly occurs at C bases linked by CpG dinucleotides. CpG exists in two forms, one is scattered in DNA highly or moderately repetitive sequences, such as Alu; the other is highly aggregated to form CpG islands (abbreviated as CGI), which are located in the 5' end promoter region of the gene, and can also be Extends into the exon region of the gene. Studies have shown that the methylation of the promoter region of the genome is one of the important molecular changes that lead to ca...

AI Technical Summary

Problems solved by technology

The advantage of this method is that it uses public large sample data for machine learning algorithm and model construction, which can be accurate to the site, and the sensitivity and specificity in existing samples are ideal; the disadvantage is that the methylation 4

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