Identification and application methods for MC1R gene haplotype

Abstract

The invention belongs to the technical field of animal husbandry or animal genetic breeding and discloses identification and application methods for an MC1R gene haplotype. The identification method for the MC1R gene haplotype comprises the steps that an MC1R gene is subjected to PCR amplification, and single-ended Sanger sequencing is adopted for obtaining a variation sequence; based on the established haplotype, the genotype and phenotype of the hair color of a breeding group are subjected to association analysis, and the haplotype for controlling a black feather and the black feather homozygous genotype are identified. According to the identification method, the haplotype for controlling the black feather and the black feather homozygous genotype can be accurately identified. According to the identification method, key SNPs loci are simultaneously identified in one sequencing reaction; but in the prior art, two sequencing primers are used for simultaneously identifying the SNPs loci. Compared with a PCR-RFLP identification method in the prior art, through Sanger sequencing, the identification accuracy is further improved.

Description

technical field [0001] The invention belongs to the technical field of animal husbandry or animal genetic breeding, and in particular relates to an identification and application method of MClR gene haplotype. Background technique [0002] At present, the closest prior art: in domestic consumer market to colored feather live chicken, the chicken of black feather is deeply liked by consumers, occupies certain market share. Feather color is a complex trait, chicken feather color is black (Extended black), golden feather (buttercup) and camellia color (wild type) by E, e bc , e + The three loci are controlled separately, and the relationship between the three loci is E>e bc >e + . Since the homozygous genotype and the heterozygous genotype of the black feather dominant locus have the same phenotype, in the breeding process, it is necessary to conduct a test cross test between the individual to be tested and the recessive homozygous individual, according to whether the...

AI Technical Summary

Problems solved by technology

[0007] (1) In the prior art, after performing PCR amplification on the MC1R gene, single-end Sanger sequencing was used to obtain the variant sequence, and based on the constructed haplotype, the correlation analysis was performed on the genotype and phenotype of the coat color of the breeding group, resulting in The haplotypes controlling black feathers and the homozygous genotypes of black feathers cannot be accurately identified

[0008] (2) The black feather chicken breeding test cross test needs to spend a lot of manpower and material resources, and the test time is long

[0009] (3) There are a large number of genetic variations in the MC1R gene of domestic local chicken breeds, and it is impossible to distinguish homozygous and heterozygous individuals in black feathers

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